EFFECT OF AUXINS AND THE BIOSTIMULATOR ALGAMINOPLANT ON RHIZOGENESIS IN STEM CUTTINGS OF TWO DOGWOOD CULTIVARS (Cornus alba AUREA AND ELEGANTISSIMA )

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1 At Si. Pol., Hortorum Cultus (), 9- EFFECT OF AUXINS AND THE BIOSTIMULATOR ALGAMINOPLANT ON RHIZOGENESIS IN STEM CUTTINGS OF TWO DOGWOOD CULTIVARS (Cornus l AUREA AND ELEGANTISSIMA ) Andrzej Pholzk, Wiesłw Szydło, Ewelin Jygrd, Mriusz Federowiz Wrsw University of Life Sienes SGGW Astrt. The UE-imposed restritions on the mnufture nd pplition of plnt protetion hemils impose on the nurserymn the need to sreen for new sustnes tht re environmentlly friendly nd yet effetive in the prodution of plnt mteril. Biopreprtions my onstitute suh group s they ontin sustne little ffeting the environment. The im of the work ws to evlute effets of the iopreprtion Algmino- Plnt on rooting of stem uttings in Cornus l Aure nd C. l Elegntissim. Freshly hrvested uttings were spryed with the.% wter solution of the iopreprtion. To ompre its effetiveness with the ommeril rooting powders used routinely in the nursery prodution prt of the uttings were treted with Ukorzeniz AB (.% NAA) or Rhizopon AA (% IBA). Both powders little ffeted rhizogenesis. Applition of the iopreprtion inresed perentge nd in dogwood s ompred to ontrol uttings wht shows its potentil in ommeril prodution. The ove tretment inresed hlorophyll, rohydrte ontents nd free mino ids in leves of the spryed uttings. Key words: iopreprtions, IBA, uttings, rooting, hlorophyll, rohydrtes INTRODUCTION The UE-imposed restritions on the mnufture nd pplition of plnt protetion hemils impose on the nurserymn the need to sreen for new sustnes tht re environmentlly friendly nd yet effetive in the prodution of plnt mteril. Biopreprtions my onstitute suh group s they ontin sustne little ffeting the environment. Biostimulnts indue plnt resistne to different stresses nd they n lso Corresponding uthor Adres do korespondenji: Andrzej Pholzk Deprtment of Ornmentl Plnts, Wrsw University of Life Sienes SGGW, ul. Nowoursynowsk 9, -787 Wrszw, Polnd, e-mil: pholzk@pozt.onet.pl; ndrzej_pholzk@sggw.pl

2 9 A. Pholzk, W. Szydło, E. Jygrd, M. Federowiz positively ffet rhizogenesis nd the root system development [Pruszyński 8]. They re either single or multi-ompound preprtions, ontining extrts from se lgs (Eloni mxim, Lminri digitt, Fuus vesiulosus or Durville pottorum) [Dorzński et l. 8]. Suh iopreprtions s AlgPlnt, Kelpk or AlgminoPlnt my ontin low moleulr polypeptides nd mino ids, vitmins, enzymes, phytohormones, sugrs, etins nd ntioxidnts [Bsk 8]. These tive ompounds my stimulte rhizogenesis y induing ntomil nd morphologil hnges in plnts [Cmri et l. 8]. Our previous results showed tht ertin iopreprtions not only enhned rhizogenesis in uttings of ornmentl shrus ut ould even reple the rooting powders [Jygrd nd Pholzk, Pholzk et l. ]. The im of this work ws to ompre iologil preprtion AlgminoPlnt with two ommeril rooting powders ontining syntheti uxins, ommonly used in nurseries. Its effet ws heked on two ultivrs of the populr ornmentl shru dogwood (Cornus l L.). An ttempt ws lso mde to detet reltionship etween severl different iohemil prmeters of uttings, s influened y tretments, nd the susequent rhizogenesis MATERIAL AND METHODS The experiments were rried out in 9 nd in ommeril nursery of M.M. Kryt in Wol Prżmowsk on two ultivrs of Cornus l Aure nd Elegntissim. In oth yers the single node uttings were prepred from shoots freshly hrvested from stok plnts on July. For the type of uttings used in the trils this dte ws found optiml in preliminry experiments rried out erlier. Leves on uttings were ut in hlf to redue trnspirtion during rooting. AlgminoPlnt ws used s.% wter solution. A pressure hnd spryer ( dm - volume) ws used to spry the uttings with the iostimultor solution until run-off. The uttings were spryed one (July ), twie (July nd 8) or three times (July, 8 nd ), depending on tretment (t. ). Auxins were pplied in the form of ommerilly ville rooting powders: Ukorzeniz AB ontining.% NAA or Rhizopon AA (% IBA), diretly to the ses of uttings. Beuse of the poor results otined in 9 with Ukorzeniz AB nd its unertin legisltive sitution this rooting powder ws omitted from the seond-yer experiment. The seond powder (Rhizopone AA) remined s it registrtion dte expires lter thn tht of Ukorzeniz nd it ontins IBA the uxin of less drsti tion. Sprying uttings with the wter solution of IBA ( mg dm - ) ws introdued insted s nother referene tretment to ompre the iopreprtion effiieny. This tretment llowed to evlute s well the IBA tion pplied in different, more sfe wy s folir pplition nd not s powder Cuttings were rooted in styrofom oxes. They were inserted to depth of m into mixture of pet, perlite nd snd (::), ph.. Rooting took ple in plsti tunnels equipped with utomti wtering nd mist systems s well s with shding devies. Every two weeks, the uttings were spryed ginst Botrytis with.% Rovrl or Sumilex. The experiment onsisted of 8 tretments (t. ), eh in replitions, nd eh replition ontining uttings. Perentge of rooted uttings nd the degree of At Si. Pol.

3 Effet of uxins nd the iostimultor AlgminoPlnt on rhizogenesis in stem uttings... 9 rooting ws determined fter 6 weeks from the eginning of the rooting experiment, i.e. on August 8. The ws evluted on -point sle desriing the development of the root ll (t. ). The sores for the represent mens of three independent oservtions y trined personnel. Tle. A list of tretments in the experiments Tel. Sposó trktowni sdzonek w doświdzenih No. of tretment Nr kominji Methods of uttings tretment Sposó trktowni sdzonek * Control Kontrol "" (H O) * Rhizopon AA (% IBA) powder puder Rhiz. AA (% IBA) + spryings with AlgminoPlnt.% Rhiz. AA (% IBA) + opryskiwnie.% preprtem AlgminoPlnt Ukorzeniz AB (.% NAA) powder puder Ukorzeniz AB (.% NAA) + spryings with AlgminoPlnt.% Ukorzeniz AB (,% NAA) + opryskiwnie.% preprtem AlgminoPlnt 6* sprying with AlgminoPlnt.% opryskiwnie,% AlgminoPlnt 7* spryings with AlgminoPlnt.% opryskiwnie,% AlgminoPlnt 8* spryings with AlgminoPlnt.% opryskiwnie,% AlgminoPlnt 9* sprying with IBA mg dm - 9 tretments: 8; tretments*,, 6, 7, 8, 9 Tle. Evlution sle of the root development Tel. Skl oeny onityjnej stopni ukorzenieni sdzonek No. Nr Cutting without visile roots Sdzonk ez widoznyh korzeni. A few shorts roots Kilk krótkih korzeni. Chrteristi of the Chrkterystyk stopni ukorzenieni Roots with numerous rnhed roots, no root ll Korzenie liznie rozgłęzione, rk ryły korzeniowej An verge root system forming root ll Średniej wielkośi system korzeniowy tworząy ryłę korzeniową Well developed, rnhed root system forming root ll Dorze wyksztłony, rozgłęziony system korzeniowy, tworząy ryłę korzeniową. Sore Liz punktów In oth yers smples for iohemil nlyses (twenty uttings per tretment) were olleted on July 8 from the ontrol untreted uttings nd those spryed three times with AlgminoPlnt. They were finely hopped, thoroughly mixed nd. g smples Hortorum Cultus ()

4 96 A. Pholzk, W. Szydło, E. Jygrd, M. Federowiz were tken for the mesurements of dry mtter, totl hlorophyll, reduing sugrs nd free mino id ontents. Triplite extrts were prepred for eh nlysis nd three mesurements were done for eh extrt giving nine redings for eh dt point. For the dry mtter ontent, g smples were dried t o C to onstnt weight [Strzelek et l. 98]. Chlorophyll ontent ws nlyzed ording to Morgn nd Porth [98] with the modifition of Inskeep nd Bloom [98]. Reduing sugrs were determined y the olorimetri method of Somogyi in the Nelson s modifition [Nelson 9] nd free mino ids were mesured ording to Rosen [97]. To ompre the mens, rooting perentges were trnsformed ording to Bliss [Wójik nd Ludński 989] while the y root trnsformtion: y = x + (x + ) sujeted to ANOVA nd tested y the Dunn s test t α =.. Results of nlyses were sujeted to the -ftoril ANOVA nd the mens were ompred y the Dunn s test t α =.. RESULTS A single-ftoril nlysis of vrine showed signifint effet of the tretments in 9 on root ll development nd perentge of rooted uttings in oth dogwood ultivrs: Aure nd Elegntissim. C. l Aure. Less thn % of the untreted ontrol uttings produed roots during 6 weeks of the experiment nd their root lls were not formed. Applition of the ommeril powders did not enhned rhizogenesis, on the ontrry, Rhizopon AA signifintly deresed perentge of rooted uttings. A joint pplition of the powders nd the iopreprtion AlgminoPlnt slightly ut signifintly improved the rooting: perentge of the rooted uttings in se of Rhizopon AA nd the in se of Ukorzeniz AB. The est effets were oserved when the iopreprtion ws used lone, espeilly if pplied twie on uttings: this tretment inresed the perentge of rooted uttings to nerly 8%, positively ffeting s well the root ll forming. All three tretments with AlgminoPlnt were etter thn the ommeril powders (fig. ). In the positive effet of Algminoplnt on rhizogenesis ws onfirmed (fig. ): the est perentge of rooting ws otined fter the twofold sprying with the iopreprtion douled s ompred to the ontrol uttings. This tretment signifintly improved s well the root ll development (expressed s ), similrly s tretments with IBA either in rooting powder or y sprying uttings with the uxin wter solutions. C. l Elegntissim. In 9 the ontrol, untreted uttings of this ultivrs rooted in 7% produing severl roots ut similrly s in Aure their root lls were not formed during 6 weeks (fig. ). No signifint effets were oserved due to the use of rooting powders, neither in perentge of rooted uttings nor in. The joint pplition of powders nd the iopreprtrion did not improved the effiieny of powders. Two- nd three-fold sprying with AlgminoPlnt enhned rhizogenesis nd signifintly inresed the perentge of rooted uttings s ompred oth to the ontrol uttings nd those treted with the ommeril rooting powders. The At Si. Pol.

5 Effet of uxins nd the iostimultor AlgminoPlnt on rhizogenesis in stem uttings perentge of rooting Control Rhizopon AA Rhizopon AA+xAlg Ukorzeniz AB Ukorzen.AB+ Alg d xalgp e xalgp d de xalgp 8 6 perentge of rooting Fig.. Effet of iostymultor AlgminoPlnt nd two rooting powders on the nd perentge of rooted uttings in Cornus l Aure (9). The mens represented y rs indited with the sme letters do not differ signifintly t α =.. Rys.. Wpływ iostymultor AlgminoPlnt i dwóh ukorzenizy n stopień i proent ukorzenieni sdzonek Cornus l Aure (9). Średnie oznzone tą smą literą nie różnią się istotnie przy α =,. perentge of rooting Control Rhizopon AA IBA xalgp d xalgp xalgp 8 6 perentge of rooting Fig.. Effet of iostymultor AlgminoPlnt nd IBA on the nd perentge of rooted uttings in Cornus l Aure (). The mens represented y rs indited with the sme letters do not differ signifintly t α =.. Rys.. Wpływ iostymultor AlgminoPlnt i IBA n stopień i proent ukorzenieni sdzonek Cornus l Aure (). Średnie oznzone tą smą literą nie różnią się istotnie przy α =,. Hortorum Cultus ()

6 98 A. Pholzk, W. Szydło, E. Jygrd, M. Federowiz perentge of rooting Control Rhizopon AA Rhizopon AA+xAlg Ukorzeni z AB Ukorzen.AB +Alg xalgp xalgp xalgp 8 6 perentge of rooting Fig.. Effet of iostymultor AlgminoPlnt nd two rooting powders on the nd perentge of rooted uttings in Cornus l Elegntissim (9). The mens represented y rs indited with the sme letters do not differ signifintly t α =.. Rys.. Wpływ iostymultor AlgminoPlnt i dwóh ukorzenizy n stopień i proent ukorzenieni sdzonek Cornus l Elegntissim (9). Średnie oznzone tą smą literą nie różnią się istotnie przy α =,. perentge of rooting 8 6 perentge of rooting Control Rhizopon AA IBA xalgp xalgp xalgp Fig.. Effet of iostymultor AlgminoPlnt nd IBA on the nd perentge of rooted uttings in Cornus l Elegntissim (). The mens represented y rs indited with the sme letters do not differ signifintly t α =.. Rys.. Wpływ iostymultor AlgminoPlnt i IBA n stopień i proent ukorzenieni sdzonek Cornus l Elegntissim (). Średnie oznzone tą smą literą nie różnią się istotnie przy α =,. At Si. Pol.

7 Effet of uxins nd the iostimultor AlgminoPlnt on rhizogenesis in stem uttings three-fold sprying with the iopreprtion resulted s well in the est root development. In the positive effet of Algminoplnt on rhizogenesis ws onfirmed: its two- nd threefold pplition signifintly improved s ompred to the ontrol uttings nd those treted with the powder while the perentge of rooted uttings ws inresed only due to threefold sprying (fig. ). In this seson lso the syntheti uxin gve good results pplied either s sprying uttings with the IBA wter solution (oth prmters mesured) or pplying the rooting powder to the uttings ses (the perentge of rooting). Biohemil nlyses. Anlysis of vrine showed signifint effets of the iopreprtion on ontents of severl orgni ompounds determined in leves of uttings during rooting (t. ). The yellow-leved ultivr Aure hd lower initil ontent of the hlorophyll thn the green Elegntissim nd the tretment with AlgminoPlnt signifintly inresed its pigment level while no effet of the iopreprtion ws seen in the ltter ultivr. Tle. Effet of iostimultor AlgminoPlnt on ontent of severl orgni ompounds in uttings of two ultivrs of Cornus l (9, ) Tel. Wpływ iostymultor AlgminoPlnt n zwrtość związków orgniznyh w sdzonkh dwóh odmin Cornus l (9, ) Compound Speies 9 Cultivr Control AlgminoPlnt Control AlgminoPlnt Chlorophyll C.. Aure.7*.7.*. mg/g d.w C.. Elegntissim d Reduing sugrs C.. Aure mg/g d.w C.. Elegntissim d Free mino ids C.. Aure μmol/g d.w C.. Elegntissim mens for given ompound followed y the sme letter do not differ signifintly t the proility level α =. wrtośi dl podnego związku poprzedzone tą smą literą nie różnią się istotnie n poziomie prwdopodoieństw α =, Also the initil level of reduing sugrs ws higher in the green-leved ultivr s ompred to Aure, however oth ultivrs responded to the tretment with the inreses in sugr ontents. The rnge of this inrese ws slightly higher in Aure (%) thn in Elegntissim (%). The tretment with the iopreprtion deresed ontents of free mino ids in oth ultivrs. Generlly, leves of uttings rooted in ontined less orgni ompounds thn in 9 ut the tendenies in their hnges resulting from the pplition of AlgminoPlnt were similr s yer efore. The hlorophyll ontent in the yellowed-lefed Hortorum Cultus ()

8 A. Pholzk, W. Szydło, E. Jygrd, M. Federowiz ultivr Aure more thn douled fter the sprying, remining on the sme level in oth tretments in the green-leved Elegntissim. Similrly s in 9, the inrese in reduing sugrs ourred in oth tretments nd ws gin more pronouned in Aure. The sme phenomenon ws oserved in ontents of free mino ids (t. ). DISCUSSION Biostimulnts ontin the iologilly tive sustnes whih positively ffet plnt growth nd development. They improve plnt tolerne to the unfvourle environmentl onditions nd ertin my ffet development of root system [Jnkowski nd Duis 8]. Extrt from se weeds re eing used in hortiulturl prodution to improve quntity nd qulity of yielding [Jouert nd Lefrn 8, Mtysik et l. 8, Rthor et l. 9]. Reently, there is growing interest in pplying iopreprtions mde from se lgs to stimulte rooting of uttings. Studies undertken y Stirk nd Stden [997] on effet of six preprtions mde from se lgs onfirmed their effiieny in improving rhizogenesis in uttings. Aording to the ove uthors the preprtions suh s Kelpk or Sem n deliver to uttings the suffiient mounts of uxins neessry to indue roots. Presene of uxins in the extrts from se weeds n qulify them s preprtions enhning rhizogenesis. Crouh nd Stden [99] lim tht most of the lgs used to produe iopreprtions ontin uxins or the uxin-likesustnes ut their onentrtions re vrile. Trils on the effet of seweed extrt on growth of Vign sinensis showed its positive effet on length nd mss of roots [Whphm et l. 6]. Thorsen et l. [] tested n effet of the extrt from n lg ontining rown pigment (Lminri digitt) on germintion, rooting nd growth of severl hereous plnts nd proved its stimultory effet on rooting of uttings ut rnge of response depended on plnt speies nd onentrtion of the preprtion. A similr plnt response ws oserved in this tril where AlgminoPlnt signifintly improved rhizogenesis in two dogwood ultivrs ut the effets depended on numer of spryings. The pplition of AlgminoPlnt resulted in n inrese in the hlorophyll ontent in the yellow-leved ultivr. Similr results were otined y Whphmt et l. [6] in their tril on tomto treted with the extrt from Asophyllum nodosum: the inreses in the hlorophyll ontents were different in different ultivrs nd depended on preprtion onentrtion. Results of Blunden et l. [996] onfirm the inrese in the green pigment level in severl rops treted with the ove se weed extrt. Similr inrese in the hlorophyll ontent ws oserved fter pplition of the extrt from Lminri digitt [Jouert nd Lfrn 8]. Sivsnkri et l. [6] report on signifint effets of iopreprtions on ontents of reduing nd totl solule sugrs in plnts. AlgminoPlnt inresed the totl nd reduing sugr levels in rrot roots [Dorzński t l. 8]. Similrly, Rthore et l. [9] oserved n elevted level of rohydrtes in plnts of Glyine mx treted with the extrt from se weed Kppphyus lvrezii. Also in oth dogwood ultivrs under study the ontents of reduing sugrs inresed fter sprying with Algmino- Plnt. Thus, the positive effet of the iopreprtion on rhizogenesis ould hve een At Si. Pol.

9 Effet of uxins nd the iostimultor AlgminoPlnt on rhizogenesis in stem uttings... relted to the improved photosynthesis resulting in n inrese in the sugr ontent in oth ultivrs nd n elevted hlorophyll level in the yellow-leved txon. This speultion is supported y the ft tht the rnge of response to the tretment with AlgminoPlnt ws lrger in the yellow-leved ultivr, ontining less hlorophyll nd reduing sugrs t the eginning of the experiment. Amino ids ply different roles in plnt orgnisms, serving minly s uilding mteril for synthesis of peptides nd proteins. Some of them re storge forms of mine nitrogen, others serve s trnsporttion forms etween different plnt prts [Kopewiz nd Lewk ]. Lesuffleur et l. [7] proved tht n elevted onentrtion of free mino ids in rhizosphere positively ffets rhizogenesis wht ould e true lso in se of Cornus. Differenes in solute vlues of the ove nlyzes etween two yers n e explined y n effet of the environmentl onditions ffeting stok plnts resulting in different physiologil sttus of uttings on the dte of their hrvest. However, oth the differenes etween the ultivrs nd rnge of their response to the tretment re of omprle mgnitude order. Dogwood uttings hve reltively high rooting ility (% nd 7% in the ultivrs used in this tril) wht my explin the lk of response fter the pplition of the routinely used rooting powders ontining uxins. Yet, the positive effets of AlgminoPlnt on the perentge of rooted uttings nd the root ll development my in future e trnslted into eonomi profits of the growers justifying the use of this iopreprtion in ommeril prodution of dogwood. CONCLUSIONS. The iostymultor AlgminoPlnt positively ffeted rhizogenesis in dogwood though its effet differed in two ultivrs under study.. The tretment with the AlgminoPlnt inresed ontents of hlorophyll +, reduing sugrs nd free mino ids in leves of uttings wht ould explin its positive effet on rhizogenesis.. Sprying dogwood uttings with the AlgminoPlnt solution n e n effetive nd environmentlly friendly method to stimulte rooting. REFERENCES Bsk A., 8. Klsyfikj iostymultorów n podstwie opinii dzy orz firm hemiznyh krjowyh i zgrniznyh. Wieś Jutr,. Blunden G., Jenkins T., Liu Y.-W., 996. Enhned lef hlorophyll levels in plnts treted with seweed extrt. J. Appl. Phyol. 8,. Cmri D., Filippini L., Apone F., Ariello S., Colui G., Portoso D., 8. Effet of Amino- Plnt on expression of seleted genes in Aridopsis thlin L. plnts. [In:] Biostimultors in modern griulture. Generl Aspets. Wieś Jutr, Wrszw, 77 8 Crouh I.J., Vn Stden J., 99. Evidene for the presene of plnt growth regultors in ommeril seweed produts. Plnt Growth Reg., 9. Hortorum Cultus ()

10 A. Pholzk, W. Szydło, E. Jygrd, M. Federowiz Dorzński A., Anyszk Z., Elkner K., 8. Rekj mrhwi n ekstrkty pohodzeni nturlnego z lg z rodzju srgssum AlgminoPlnt i z leonrdytu HumiPlnt. J. Res. Appl. Agri. Engin., (), 8. Inskeep, W.P., Bloom P.R., 98. Extintion oeffiients of hlorophyll nd in N,Ndimethylformmide nd 8% etone. Plnt Physiol. 77, 8 8 Jygrd E., Pholzk A.,. Effet of iopreprtions Amino Totl nd Biohikol PC on rhizogenesis in stem uttings of Physorpus opulifolius Drt s Gold nd Diolo. Ann. WULS, 9 8. Jnkowski K., Duis B., 8. Biostymultory w polowej produkji roślinnej. Biostymultory w nowozesnej uprwie roślin. Plntpress, 7 8 lutego 8, Wrszw,. Jouert J.M., Lefrn G., 8. Njnowsze dni nd fitostymultormi pozyskiwnymi z glonów morskih. Dw rodzje produktów stymultory wzrostu i odżywini orz stymultory rekji oronnyh. Biostymultory w nowozesnej uprwie roślin. Plntpress, 7 8 lutego 8, Wrszw, 6. Kopewiz J., Lewk S.,. Fizjologi roślin. PWN, Wrszw. Lihtenthler H.K., Wellurn A.R., 98. Determintions of totl rotenoids nd hlorophyll nd of lef extrts in different solvents. Biohem. So. Trns. 6, 9 9. Lessufleur F., Pynel F., Btillem P., 7. Root mino id exudtion: mesurement of high efflux rtes of glyine nd serine from six different plnt speies. Plnt nd Soil 9, 6. Nelson N., 9. A photometri dpttion of the Somogyi method for the determintion of gluose. J. Biol. Chem., 7 8. Mtysik K., Kzmrek S., Admzewski K., 8. Zstosownie ioregultor Kelpk w roślinh uprwnyh. Biostymultory w nowozesnej uprwie roślin. Plntpress. 7 8 lutego 8, Wrszw, 6. Morgn R., Porth D., 98. Chlorophyll determintion in intt tissues using N,N-Dimethylformmide. Plnt Physiol. 6, Pholzk A., Szydło W., Jygrd E.,. Wpływ preprtów Amino Totl i Biohikol PC n ukorzeninie sdzonek pędowyh Bereris thunergii i Cornus l. Zesz. Pro. Post. Nuk Roln., 9 9. Pruszyński S., 8. Miejse iostymultorów w ohronie roślin i nwożeniu. Wieś Jutr,. Rthore S.S., Chudhry D.R., Borih G.N., Gosh A., Bhtt B.P., Zdope S.T., Ptoli J.S., 9. Effet of seweed extrt on growth, yield nd nutrient uptke of soyen (Glyine mx) under rinfed onditions. South Afrin J. Bot. 7,. Rosen H., 97. A modified ninhydrin olurimetri nlysis for mino ids. Arh. Biohem. Bioph. 67,. Sivsnkri S., Venkteslu V., Annthrj M., Chndrsekrn M., 6. Effet of seweed extrts on the growth nd iohemil onstituents of Vign sinensis. Bioresoure Teh. 97, 7 7. Stirk W.A., Vn Stden J., 997. Comprison of ytokinin- nd uxin-like tivity in some ommerilly used seweed extrts. J. App. Phyol. 8, 8. Strzelek H., Kmińsk., Kowlski J., 98. Chemizne metody dń roślinnyh surowów leznizyh. PZWL, Wrszw, 6 7. Thorsen M.K., Woodwrd M., MKenzie B.M.,. Kelp (Lminri digitt) inreses germintion nd ffets rooting nd plnt vigour in rops nd ntive plnts from n rle grsslnd in the Outer Herides, Sotlnd. J. Costl Conserv. Pulished online: 6 Ferury. Whphm C.A., Blunden G., Jenkins T., Hnkins S.D., 6. Signifine of etines in the inresed hlorophyll ontent of plnts treted with seweed extrt. J. App. Phyol.,. At Si. Pol.

11 Effet of uxins nd the iostimultor AlgminoPlnt on rhizogenesis in stem uttings... Wójik A. R., Ludński Z., 989. Plnownie i wnioskownie sttystyzne w doświdzlnitwie. PWN, Wrszw,. WPŁYW AUKSYN I PREPARATU ALGAMINOPLANT NA UKORZENIANIE SADZONEK PĘDOWYCH DWÓCH ODMIAN DERENIA BIAŁEGO (Cornus l AUREA I ELEGANTISSIMA ) Streszzenie. Wprowdzne przez UE ogrnizeni produkji i zstosowni środków ohrony roślin wymuszją n szkółkrzh poszukiwni nowyh sustnji ezpieznyh dl środowisk skuteznyh w produkji mteriłu roślinnego. Celem niniejszej pry yło określenie wpływu preprtu z grupy iostymultorów AlgminoPlntu zstosownego w formie opryskiwni sdzonek.% roztworem wodnym, n proes ukorzenini sdzonek pędowyh Cornus l Aure i Elegntissim. Dl porównni użyto stosowne dotąd w prktye szkółkrskiej ukorzenize pudrowe: Ukorzeniz AB (.% NAA) i Rhizopon AA (% IBA). Zstosowne ukorzenize w niewielkim stopniu oddziływły n rizogenezę u dereni. Wyniki przeprowdzonego doświdzeni wskzują ntomist n możliwość wykorzystni preprtu AlgminoPlnt w proesie ukorzenini w/w odmin dereni, poniewż jego zstosownie zwiększyło proent i stopień ukorzenieni sdzonek w porównniu z sdzonkmi kontrolnymi. Preprt ten wpłynął n wzrost zwrtośi hlorofilu, węglowodnów zwrtość i wolnyh minokwsów w liśih sdzonek. Słow kluzowe: iostymultory, IBA, sdzonki, ukorzeninie, hlorofil, ukry redukująe Aepted for print Zkeptowno do druku:.. Hortorum Cultus ()