THE QUANTITATIVE ANALYSIS OF POLIPHENOLIC COMPOUNDS IN DIFFERENT PARTS OF THE ARTICHOKE (cynara scolymus L.) DEPENDING ON GROWTH STAGE OF PLANTS

Podobne dokumenty
Międzynarodowa Konferencja Naukowa

ZALEŻNOŚĆ MIĘDZY NORMĄ WYSIEWU NASION A PLONEM ZIELA KARCZOCHA (CYNARA SCOLYMUS L.) * Wstęp. Materiał i metody

The estimation of the yield and content of some chemical compounds in the fruits of chosen hot pepper (Capsicum annuum L.

CONTENT OF PHENOLIC ACIDS IN EDIBLE PARTS OF SOME ALLIUMS SPECIES GROWN FOR THE GREEN BUNCHING

OCENA PLONOWANIA ZIELA KARCZOCHA ZWYCZAJNEGO (CYNARA CARDUNCULUS L. SSP. FLAVESCENS WIKL.) W WARUNKACH ZRÓŻNICOWANEGO NAWOŻENIA MINERALNEGO

Cracow University of Economics Poland. Overview. Sources of Real GDP per Capita Growth: Polish Regional-Macroeconomic Dimensions

THE INFLUENCE OF SELECTED FACTORS ON THE YIELD OF Allium moly L. BULBS. Jerzy Hetman, Halina Laskowska, Wojciech Durlak

Has the heat wave frequency or intensity changed in Poland since 1950?

Is there a relationship between age and side dominance of tubal ectopic pregnancies? A preliminary report

WZROST I PLONOWANIE PAPRYKI SŁODKIEJ (CAPSICUM ANNUUM L.), UPRAWIANEJ W POLU W WARUNKACH KLIMATYCZNYCH OLSZTYNA


Nauka Przyroda Technologie Dział: Ogrodnictwo

Evaluation of selected quality traits of storage roots of ten beet cultivars

PRELIMINARY ASSESSMENT OF THE QUALITY OF HERBHONEYS AND CHOKEBERRY SYRUPS USED FOR THEIR PRODUCTION

Effect of cultivar on early yield of parsley grown from the late summer sowing

Thermooxidative stability of frying oils and quality of snack products

WZROST I ROZWÓJ FREZJI UPRAWIANEJ W GRUNCIE W ZALEŻNOŚCI OD TERMINU SADZENIA. Wstęp


Krytyczne czynniki sukcesu w zarządzaniu projektami

Charakterystyka kliniczna chorych na raka jelita grubego

Setting of southern sweet-grass plantation with stem cuttings obtained by division of maternal plants

TRANSPORT W RODZINNYCH GOSPODARSTWACH ROLNYCH

OCENA PLONOWANIA ODMIAN BURAKA LIŚCIOWEGO W UPRAWIE JESIENNEJ. Wstęp. Materiał i metody

AKADEMIA MORSKA W SZCZECINIE WYDZIAŁ MECHANICZNY ROZPRAWA DOKTORSKA. mgr inż. Piotr Smurawski

Fizyka Procesów Klimatycznych Wykład 11 Aktualne zmiany klimatu: atmosfera, hydrosfera, kriosfera

WPŁYW OSŁON ORAZ SPOSOBU SADZENIA ZĄBKÓW NA PLONOWANIE CZOSNKU W UPRAWIE NA ZBIÓR PĘCZKOWY. Wstęp

Machine Learning for Data Science (CS4786) Lecture 11. Spectral Embedding + Clustering

Effect of mineral fertilization on yielding of spring false flax and crambe

DOI: / /32/37

Nauka Przyroda Technologie

Seasonal variation of phenolics content in above- and underground organs of dropwort (Filipendula vulgaris Moench)

SSW1.1, HFW Fry #20, Zeno #25 Benchmark: Qtr.1. Fry #65, Zeno #67. like


Pro-tumoral immune cell alterations in wild type and Shbdeficient mice in response to 4T1 breast carcinomas

The influence of shading on the yield and quality of southern sweet-grass (Hierochloë australis (Schrad.) Roem. & Schult.

DIFFERENCES IN CADMIUM AND LEAD ACCUMULATION BY LETTUCE (Lactuca sativa L.) DEPENDING ON THE CULTIVAR

PROTEIN CONTENT IN MIXTURES OF BLUE LUPINE WITH OAT GROWN FOR GREEN FORAGE. Anna Płaza, Barbara Gąsiorowska, Artur Makarewicz

Sargent Opens Sonairte Farmers' Market

Unit of Social Gerontology, Institute of Labour and Social Studies ageing and its consequences for society

Patients price acceptance SELECTED FINDINGS

Intraspecific variability of great burnet (Sanguisorba officinalis L.) in respect of sterols content

EFFECT OF A SOWING DATE ON THE QUANTITY AND QUALITY OF THE YIELD OF TARRAGON (ARTEMISIA DRACUNCULUS L.) GROWN FOR A BUNCH HARVEST

Yield structure of seven strawberry cultivars

Ocena skuteczności preparatów miejscowo znieczulających skórę w redukcji bólu w trakcie pobierania krwi u dzieci badanie z randomizacją

Lek. Ewelina Anna Dziedzic. Wpływ niedoboru witaminy D3 na stopień zaawansowania miażdżycy tętnic wieńcowych.

THE ESTIMATION OF BIOACTIVE COMPOUNDS CONTENT IN ORGANIC AND CONVENTIONAL SWEET CHERRY (Prunus avium L.)

TYRE PYROLYSIS. REDUXCO GENERAL DISTRIBUTOR :: ::

Nauka Przyroda Technologie

INSTYTUT GENETYKI I HODOWLI ZWIERZĄT POLSKIEJ AKADEMII NAUK W JASTRZĘBCU. mgr inż. Ewa Metera-Zarzycka

FIRMNESS AND ANTIOXIDANT CAPACITY OF HIGHBUSH BLUEBERRY (VACCINIUM CORYMBOSUM L.) GROWN ON THREE TYPES OF ORGANIC BED

NUTRITION STATUS OF GREENHOUSE TOMATO GROWN IN INERT MEDIA. Part II. MICROELEMENTS

Tytuł pracy w języku angielskim: Physical properties of liquid crystal mixtures of chiral and achiral compounds for use in LCDs

KOMBAJNY ZBOŻOWE W ROLNICTWIE POLSKIM W LATACH

Tychy, plan miasta: Skala 1: (Polish Edition)

Robert Gruszecki * & Andrzej Sałata

CHARAKTERYSTYKA WARUNKÓW METEOROLOGICZNYCH W REJONIE DOŚWIADCZEŃ ŁĄKOWYCH W FALENTACH

THE EFFECT OF NON-WOVEN PP FABRIC COVERS ON THE YIELDING AND THE FRUIT QUALITY OF FIELD-GROWN SWEET PEPPERS

SPITSBERGEN HORNSUND

EFFECT OF VARIOUS DOSES OF NPK FERTILIZERS ON CHLOROPHYLL CONTENT IN THE LEAVES OF TWO VARIETIES OF AMARANTH (Amaranthus cruentus L.

Stargard Szczecinski i okolice (Polish Edition)

Analysis of infectious complications inf children with acute lymphoblastic leukemia treated in Voivodship Children's Hospital in Olsztyn

Ocena potrzeb pacjentów z zaburzeniami psychicznymi

BILANS ENERGETYCZNY. Wykorzystanie energii. Przepływ energii. Barbara Pietrzak, Ekologia 2018, Wydział Biologii UW

Nauka Przyroda Technologie

Latent Dirichlet Allocation Models and their Evaluation IT for Practice 2016

ZEWNĄTRZKOMÓRKOWEJ U PACJENTÓW OPEROWANYCH Z POWODU GRUCZOLAKA PRZYSADKI

Fig 5 Spectrograms of the original signal (top) extracted shaft-related GAD components (middle) and

WPŁYW NAWOŻENIA AZOTOWEGO NA PLONOWANIE I WARTOŚĆ BIOLOGICZNĄ BURAKA ĆWIKŁOWEGO, UPRAWIANEGO NA ZBIÓR PĘCZKOWY. Wstęp

Akademia Morska w Szczecinie. Wydział Mechaniczny

OpenPoland.net API Documentation

Proposal of thesis topic for mgr in. (MSE) programme in Telecommunications and Computer Science

Zakopane, plan miasta: Skala ok. 1: = City map (Polish Edition)

ZAWARTOŚĆ AZOTANÓW (V) W SAŁACIE I SZPINAKU W POLSCE W LATACH

INFLUENCE OF SUGAR BEET SEED PRIMING METHOD ON GERMINATION UNDER WATER SHORTAGE CONDITIONS AND ROOT SYSTEM DEVELOPMENT

Contents of secondary metabolites at various anatomical parts of three wild strawberry (Fragaria vesca L.) cultivars

THE EFFECT OF PROHEXADIONE-CA ON SHOOT GROWTH AND CROPPING OF YOUNG APPLE TREES OF JONAGOLD CV.

WPŁYW INTENSYWNOŚCI RĘCZNEGO PRZERZEDZANIA ZAWIĄZKÓW NA WYBRANE CECHY JAKOŚCIOWE JABŁEK ODMIANY SZAMPION

Cracow University of Economics Poland

DUAL SIMILARITY OF VOLTAGE TO CURRENT AND CURRENT TO VOLTAGE TRANSFER FUNCTION OF HYBRID ACTIVE TWO- PORTS WITH CONVERSION

Introduction. Agricultural Engineering w ww.wir.ptir.org ASSESSMENT OF SELECTED TECHNICAL PARAMETERS OF SOYA SEEDS OIL PRESSING PROCESS

ROZPRAWY NR 128. Stanis³aw Mroziñski

SPITSBERGEN HORNSUND

ZANIKANIE KAPTANU I PROPIKONAZOLU W OWOCACH I LIŚCIACH JABŁONI ODMIANY JONAGOLD


West Pomeranian University of Technology in Szczecin

ROZPRAWA DOKTORSKA. Mateusz Romanowski

Katarzyna Durda STRESZCZENIE STĘŻENIE KWASU FOLIOWEGO ORAZ ZMIANY W OBRĘBIE GENÓW REGULUJĄCYCH JEGO METABOLIZM JAKO CZYNNIK RYZYKA RAKA W POLSCE

INSPECTION METHODS FOR QUALITY CONTROL OF FIBRE METAL LAMINATES IN AEROSPACE COMPONENTS

Analiza epidemiologiczna występowania i uwarunkowań pylicy górników kopalń węgla kamiennego w województwie śląskim w latach

WYKAZ DOROBKU NAUKOWEGO

ABOUT NEW EASTERN EUROPE BESTmQUARTERLYmJOURNAL

Uniwersytet Medyczny w Łodzi. Wydział Lekarski. Jarosław Woźniak. Rozprawa doktorska

SPITSBERGEN HORNSUND

Wpływ wybranych parametrów technologicznych na zawartość estrów glicydylowych w tłuszczach i smażonych produktach

OCENA STANU WIEDZY UCZNIÓW SZKÓŁ POLICEALNYCH NA TEMAT DODATKÓW DO ŻYWNOŚCI

deep learning for NLP (5 lectures)

Raport bieżący: 44/2018 Data: g. 21:03 Skrócona nazwa emitenta: SERINUS ENERGY plc

INŻYNIERIA ROLNICZA AGRICULTURAL ENGINEERING

SPITSBERGEN HORNSUND

Transkrypt:

Acta Sci. Pol., Hortorum Cultus 9(3) 21, 175-181 THE QUANTITATIVE ANALYSIS OF POLIPHENOLIC COMPOUNDS IN DIFFERENT PARTS OF THE ARTICHOKE (cynara scolymus L.) DEPENDING ON GROWTH STAGE OF PLANTS Andrzej Sałata, Robert Gruszecki University of Life Sciences in Lublin Abstract. A diversity of active substances that are in the artichoke plants includes it into the group of medicinal plants of broad-spectrum performance. The research conducted in the years 26 28 included valuation of poliphenolic compounds content in different parts of artichoke plants during vegetative and generative growth (roots, petioles, leaves, immature and flower at beginning of flowering). The total content of poliphenolic compounds in the reduction on caffeic acid was marked in dried herb with the spectrophotometrical method with the Arnova reagent. The content of poliphenolic acids (caffeic, chlorogenic, ferulic and cynarine) was marked with high performance liquid chromatography (HPLC). The undertaken studies show that there are significant differences with respect to the content of poliphenolic compounds in different parts of artichoke plants. Definitely more total phenolic acids were accumulated in leaves during the vegetative growth (3.167% on average) and in young, immature buds during generative growth (3.73% on average). The chlorogenic acid and cynarine were the main compounds among poliphenolic acids. The content of poliphenolic acids was decreasing with age of plants as young immature artichoke buds had more chlorogenic acid and cynarine than at the beginning of flowering. The content of caffeic and ferulic acids in the artichoke herb depended on the growth phase of plants. Plants accumulated more caffeic acid in leaves during vegetative growth and ferulic acid in buds during generative growth. Key words: artichoke, Cynara scolymus L., poliphenolic compounds, phenolic acids, growth stage INTRODUCTION An artichoke (Cynara scolymus L.) is perennial thistle belongs to the Asteraceae (Compositae) family and is a commonly cultivated plant in Southern Europe around the Corresponding author Adres do korespondencji: Andrzej Sałata, Robert Gruszecki, Department of Vegetable Crops and Medicinal Plants, University of Life Sciences in Lublin, Leszczyńskiego 58, 2-68 Lublin, Poland; e-mail: andrzej.salata@up.lublin.pl

176 A. Sałata, R. Gruszecki Mediterrean Sea region [Bianco 25]. Due to the high temperature requirements, in the climate conditions of Poland artichoke is cultivated as an annual plant. The edible parts of the artichoke are immature flower buds which include thick, fleshy heart (base of a head) together with immature floret scales (involucral bracts). The artichoke is also a valuable herbal plant. A pharmaceutical material are dried leaves (Cynarae folium) or herb (Cynarae scolymi herba). The diversity of active substances which are found in the artichoke classifies it as belonging to the group of medicinal plants having broad-spectrum pharamacological action. Substances found in the artichoke herb are assumed to effectively cure digestive and circulation disorders, they show strong antioxidant properties that protect organism against some tumours, lower the level of triglicerides and stimulate the immune system [Kirchoff et al. 1994; Kraft 1997; Llorach et al. 22]. Phenolic acids found in the artichoke improve gall blader function, lower the the LDL cholesterol fraction, strenghten and regenerate liver cells [Wójcicki et al. 1981; Sidrach et al. 25]. Compounds included in the artichoke plants have antiviral, antibacterial and antifungal properties [Llorach et al. 22; Zhu et al. 25]. According to many opinions, poliphenolic compounds found in artichoke plants, especially mono- and di-caffeoylquinic acids and flavonoids, decide about their healthenhancing and antioxidant properties [Wittemer et al. 25; Fratianni et al. 27]. The aim of the undertaken research, which results are shown in this work, was to estimate the content and chemical composition of poliphenolic compounds found in different parts of the artichoke depending on growth stage of plants. MATERIAL AND METHODS The experiment was conducted in the experimental field of the Departament of Vegetable Crops and Medicinal Plants, University of Life Sciences in Lublin while phytochemical analysis were done in the laboratory of the Department, in the years 26 28. The experimental material were artichoke plants of the Green Globe variety. The artichoke plants were cultivated from seedlings, with spacing 1. 1. m, in four replications. The transplants was planted outside in the phase 3 4 leaves in 1 st decade of May in the seasons 26 28. In each year of research 3 plants in vegetative (height around 45 cm) and generative stage (young, not developed artichoke bud and at the beginning of head flowering) were harvested. Herb was collected in the vegetative phase in the 1 st decade of July (9 days old) and generative phase: in the 1 st decade of August (12 days old) immature buds and 1 st decade of September (15 days old) flower at beginning of flowering. The whole plants were digged out and then prepared separating: roots, leaf nodes, whole leaves (node with a leaf blade) in vegetative stage and roots, leaf nodes, whole leaves, young immature buds and flower heads at the beginning of the flowering. Directly after the harvest the fresh material was dried in a drying room in the temperature of 4 C. The 1 kg sample was prepared from the air dried herb of each combination directly grinded in a mill with sieve holes of 1 mm diameter. Samples of grinded Acta Sci. Pol.

The quantitative analysis of poliphenolic compounds in different parts of the artichoke... 177 material were kept in hermetic containers for laboratory use. Chemical analysis involved dry plant material in each year of research. The total content of phenolic acids was marked of spectrometric Arnova method (according to the Farmakopea Polska 1999) and expressed as caffeic acid equivalents. The chromatographic determination of phenolic acids was performed by HPLC, using LiChrom (Merck) chromatograph equipped with DAD detector (L-745). A Lichrospher 1 RP-18 column (25 4 mm, 5µm) maintained 25 o C. Eluent comprised: (A) acetonitryle (B) H 2 O (1% acetic acid), in the following gradient: 2 min: 95% B to 64% B; 2 min to 25 min 64% B; 25 to 27 min 64% B to 95% B. The flow rate was 1 ml min -1, the iniection volume was 1 µm, and the spectra were recorded in the range 22 4 nm. The calibration curves were generated with concentration ranging from.1 to 1. mm of chlorogenic acid (5-caffeoylquinic), caffeic acid (3,4-dicaffeoylquinic), ferulic acid (4-hydro-3-methylcinnamale) and cynarin (1,3-dicaffeoylquinic). The obtained results were evaluated statistically with analysis of variance and Tukey t-test at a 5% level of significance. RESULTS AND DISCUSSION There occurred the considerable differences regarding the general content of poliphenolic compounds in different parts of plants (tab. 1). Definitely more poliphenolic acids were marked in young, immature buds of artichoke than in leaves, which were harvested from plants in vegetative and generative stage. The least biologically active substances were found in roots and leaf nodes. In an earlier research it was proved that artichoke plants accumulate more poliphenols in flower heads than in leaves [Romani et al. 26; Fratianni et al. 27]. Wide diversity of content of phenolic acids in edible parts of some alliums species observed Mysiak and Tendaj [28]. Accumulation of active substances was influenced also by a development stage that the plants were harvested in. Roots of plants and leaves in vegetative stages contained considerably more total phenolic acids than at the beginning of flowering period, while in leaf nodes more poliphenolic compounds were marked during flowering than in the vegetative stage. The dominant compound was chlorogenic acid, known to have oxireductive and anticarcinogenic properties [Rechner et al. 22]. The large content of this substance explains its important role in biochemical reactions leading to form different poliphenolic compounds [Wittemer et al. 25]. In roots, leaf nodes and leaves of plants the content of this substance was similar to the one observed before and during flowering period, but the highest amount was in leaves (vegetative stage 233 mg mg 1 g -1 dry weight; generative phase 229 mg 1 g -1 dry weight) than in other parts of the plant (fig. 1). The content of chlorogenic acid was lowered with the age of plants, as the young immature heads of artichoke had more of the substance (61 mg 1 g -1 dry weight) than mature heads at the beginning of the flowering period (26 mg 1 g -1 dry weight). No cynarin was marked in roots harvested in the vegetative and generative stage. The most cynarin was found in leaves (before flowering 156 mg 1 g -1 dry weight and during flowering Hortorum Cultus 9(3) 21

178 A. Sałata, R. Gruszecki mg 1 g -1 DM mg 1 g -1 s.m. 25 2 15 1 5 Chlorogenic acid Kwas chlorogenowy roots korzenie leaf nodes ogonki liściowe leaves liście immature buds rozwinęte mg 1 g -1 DM mg 1 g -1 s.m. 15 1 5 Cynarin Cynaryna roots korzenie leaf nodes ogonki liściowe leaves liście immature buds rozwinęte mg 1 g -1 DM mg 1 g -1 s.m. 4 35 3 25 2 15 1 5 Caffeic acid Kwas kawowy roots korzenie leaf nodes ogonki liściowe leaves liście im rozwinęte mg 1 g -1 DM mg 1 g -1 s.m. 16 14 12 1 8 6 4 2 Ferulic acid Kwas ferulowy roots korzenie leaf nodes ogonki liściowe vegetative stage faza wegetatywna leaves liście immature buds rozwinęte generative stage faza generatywna Fig. 1. Quantitative analysis of the major phenolic acids in different parts of artichoke at the vegetative and generative stage of plant growth Rys. 1. Zawartość zidentyfikowanych fenolokwasów w różnych częściach roślin karczocha w fazie wzrostu wegetatywnego i generatywnego Acta Sci. Pol.

The quantitative analysis of poliphenolic compounds in different parts of the artichoke... 179 Table 1. Total phenolic acids content (expressed as caffeic acid equivalents) in different parts of the artichoke plant (% DM) Tabela 1. Zawartość kwasów polifenolowych ogółem (w przeliczeniu na kwas kawowy) w różnych częściach rośliny karczocha (% powietrznie suchej masy) Roots Korzenie Leaf nodes Ogonki liściowe Leaves Liście Part of plant Część rośliny Immature buds Pąki Flower at beginning of flowering Kwiatostan na początku kwitnienia Years Lata Vegetative stage Faza wegetatywna Generative stage Faza generatywna 26.24a*.13b 27.7c.6c 28.14b.1b mean średnia.15a.1b 26.67d.69d 27.155c.279ab 28.245b.39a mean średnia.155b.219a 26 2.33d 2.317d 27 3.722a 3.213c 28 3.45b 2.29e mean średnia 3.167A 2.579B 26-3.746a 27-3.738a 28-3.76a mean średnia - 3.73 26-1.355a 27-1.336a 28-1.316a mean średnia - 1.335 *Means values marked with the same letters among each part of artichoke do not differ significantly among each other at probability level of α =.5. Średnie oznaczone tymi samymi literami w obrębie każdej części rośliny nie różnią się istotnie między sobą na poziomie prawdopodobieństwa α =,5. 159 mg 1 g -1 dry weight) and in young heads (53 mg 1 g -1 dry weight), while less in (12 mg 1 g -1 s.m.). Accumulation of caffeic acid and ferulic acid depended to a great degree on the plant development stage. Caffeic acid, which derivatives are used with success for insufficiency of vein circulation theraphy [Wittemer et al. 25], was found in substantial amoung in artichoke leaves, especially those harvested during vegetative stage (34 mg 1 g -1 s.m.). In roots and leaf nodes the content of this substance was not observed at all. Ferulic acid was found in small quantities. It s presence was noted only in the generative stage, in young (14 mg 1 g -1 dry weight) and mature (5 mg 1 g -1 dry weight) artichoke heads. Regarding the large diversity of poliphenolic compounds found in flower heads the interest in their use for production of health-enhancing functional food increases [Meijer and Mathijsen 1996; Romani et al. 26; Kahle et al. 27]. Hortorum Cultus 9(3) 21

18 A. Sałata, R. Gruszecki CONCLUSIONS 1. Content of phenolic acids (expressed as caffeic acid equivalents) of flowers heads as well as leaves of artichoke were high although varied depending of development stage of plants. 2. More phenolic acids were marked in immature buds of artichoke than in leaves, roots, leaf nodes as well as flowers at beginning of flowering. 3. The content of chlorogenic acid and cynarein were lowered with the age of plants, as im of artichoke had more of the substance than at the beginning of the flowering period. 4. Artichoke leaves contained more caffeic acid especially those harvested during vegetative stage. Ferulic acid was found only in artichoke heads in the generative stage. REFERENCES Bianco V.V., 25. Present situation and future potential of artichoke in the Mediterranean basin. Acta Hort. 681, 39 55. Farmakopea Polska V, 1999. Wyd. PT Farm. Warszawa, 88 881. Fratianni F., Tucci M., De Palma M., Pepe R., Nazzaro F., 27. Polyphenolic composition on different parts of some cultivars of globe artichoke (Cynara cardunculus L. var. scolymus (L.) Fiori). J. Agric. Food Chem. 14, 1282 1286. Kahle K., Huemmer W., Kempf M., Scheppach W., Erk T., Richling E., 27. Polyphenols are intensively metabolized in the human gastrointestinal tract after apple juice consumption. J. Agric. Food Chem. 55, 165 1614. Kirchhoff R., Beckers C., Kirchhoff G.M., Trinczek-Gartner H., Petrowicz O., Reiman H.J., 1994. Increase in choleresis by means of artichoke extract. Results of a randomized placebo controlled double-blind study. Phytomedicine 1, 17115. Kraft K., 1997. Artichoke leaf extract recent findings reflecting effects on lipid metabolism, liver and gastrointestinal tracts. Phytomedicine 4, 369 378. Llorach R., Espin J.C., Tomas-Barberan F.A., Ferreres F., 22. Artichoke (Cynara scolymus L.) byproducts as a potential source of health-promoting antioxidant phenolics. J. Agric. Food Chem. 5, 3458 3464. Meijer W., Mathijsen W., 1996. Analysis of crop performance in research on inulin, fibre and oil seeds crops. Ind. Crops Prod. 5, 253 264. Mysiak B., Tendaj M., 28. Content of phenolic acids in edible parts of some alliums species grown for the green bunching. Acta Sci. Pol., Hortorum Cultus 7(4), 57 62. Rechner A.R., Kuhnle G., Bremner P., Hubbard G.P., Moore K.P., Rice-Evans C.A., 22. The metabolic fate of dietary polyphenols in humans. Free Radic. Biol. Med. 33, 22 235. Romani A., Pinelli P., Cantini C., Cimato C., Heimler D., 26. Characterization of Violetto di Toscana, typical Italian variety of artichoke (Cynara scolymus L.). J. Agric. Food Chem. 95, 221 225. Sidrach L., Garcia-Canovas F., Tudela J., Rodriquez-Lopez J., 25. Purification of cynarases from artichoke (Cynara scolymus L.) enzymatic properties of cynarase. Phytochem. 66, 41 49. Wittemer S.M., Ploch M., Windeck T., Muller S.C., Drewelow B., Derendorf H., Veitd M., 25. Bioavailability and pharmacokinetics of caffeoylqunic acids and flavonoids after oral administration of Artichoke leaf extracts in humans. Phytomedicine 12, 28 38. Acta Sci. Pol.

The quantitative analysis of poliphenolic compounds in different parts of the artichoke... 181 Wójcicki J., Samochowiec K., Kośmider K., 1981. Wpływ wyciągu z karczochów (Cynara scolymus L.) na poziom lipidów w surowicy krwi ludzi w podeszłym wieku. Herba Pol. 27, 265 268. Zhu X., Zhang H., Lo R., 25. Antifungal activity of Cynara scolymus L. extracts. Fitoterapia 76, 18 111. ZAWARTOŚĆ ZWIĄZKÓW POLIFENOLOWYCH W RÓŻNYCH CZĘŚCIACH KARCZOCHA ZWYCZAJNEGO (Cynara scolymus L.) W ZALEŻNOŚCI OD STADIUM ROZWOJOWEGO ROŚLIN Streszczenie. Różnorodność substancji aktywnych występujących w karczochu włącza go do grupy roślin leczniczych o szerokim spektrum działania. Badania przeprowadzone w latach 26 28 obejmowały ocenę zawartości związków polifenolowych w różnych częściach roślin karczocha w fazie wzrostu wegetatywnego i generatywnego (korzeniach, ogonkach liściowych, liściach, pąkach kwiatostanowych nierozwiniętych i na początku kwitnienia). Zawartość kwasów fenolowych ogółem w przeliczeniu na kwas kawowy oznaczono w suchej masie ziela metodą spektrofotometryczną z odczynnikiem Arnova. Skład fenolokwasów oznaczono metodą HPLC: kawowego, chlorogenowego, feulowego, cynaryny. Z przeprowadzonych badań wynika, że występują znaczne różnice pod względem zawartości związków polifenolowych w różnych częściach rośliny. Zdecydowanie więcej kwasów fenolowych ogółem rośliny zgromadziły w liściach w fazie wzrostu wegetatywnego średnio 3,167%, oraz w fazie generatywnej w młodych nierozwiniętych pąkach średnio 3,73%. Dominującym związkiem wśród kwasów fenolowych był kwas chlorogenowy i cynaryna. Zawartość kwasów fenolowych zmniejszała się wraz z wiekiem roślin gdyż młode karczocha zawierały więcej kwasu chlorogenowego i cynaryny niż rozwinięte, na początku kwitnienia. Zawartość kwasu kawowego i ferulowego w zielu karczocha zależała od fazy rozwojowej rośliny. Więcej kwasu kawowego rośliny zgromadziły w liściach w fazie wzrostu wegetatywnego a kwasu ferulowego w pąkach w fazie wzrostu generatywnego. Słowa kluczowe: karczoch, Cynara scolymus L., związki polifenolowe, kwasy fenolowe, faza wzrostu Accepted for print Zaakceptowano do druku: 23.6.21 Hortorum Cultus 9(3) 21