Prof. dr hab. Grzegorz Węgrzyn

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1 Curriculum Vitae Prf. dr hab. Grzegrz Węgrzyn Current affiliatin and fficial address: Department f Mlecular Bilgy, Faculty f Bilgy, University f Gdansk, Wita Stwsza 59, Gdansk, Pland Tel Fax: wegrzyn@bitech.ug.edu.pl Educatin, degrees and academic titles Studies , Bilgy, specialty: Mlecular Bilgy, University f Gdansk (Pland), Faculty f Bilgy, Gegraphy and Oceanlgy MSc 1987, Bilgy, specialty: Mlecular Bilgy, University f Gdansk (Pland), Faculty f Bilgy, Gegraphy and Oceanlgy PhD 1991, Bilgical Sciences (Bilgy), specialty: Mlecular Bilgy; University f Gdansk (Pland), Faculty f Bilgy, Gegraphy and Oceanlgy Habilitatin (D.Sc.) 1995, Bilgical Sciences (Bilgy), specialty: Mlecular Genetics; University f Gdansk (Pland), Faculty f Bilgy, Gegraphy and Oceanlgy Prfessr (title; Bilgical Sciences) 1998, University f Gdansk (Pland), Faculty f Bilgy, Gegraphy and Oceanlgy Freign scientific schlarships and residences Chair f Bichemistry, University f Nttingham Medical Schl, Great Britain 1991 Mlecular Genetics Center, University f Califrnia, San Dieg, USA 1992 Career/emplyment Research Assistant , Department f Mlecular Bilgy, Faculty f Bilgy, University f Gdansk Lecturer (Adiunkt) , Department f Mlecular Bilgy, Faculty f Bilgy, University f Gdansk Assistant Prfessr , Department f Mlecular Bilgy, Faculty f Bilgy, University f Gdansk Assciate Prfessr , Department f Mlecular Bilgy, Faculty f Bilgy, University f Gdansk Prfessr d 1999, Department f Mlecular Bilgy, Faculty f Bilgy, University f Gdansk Other emplyments r functins Part time , Sea Bilgy Centre f the Plish Academy f Sciences, Oceanlgy Institute f the Plish Academy f Sciences

2 Scientific crdinatr at Labratry f Mlecular Bilgy in the Institute f Bichemistry and Biphysics f the Plish Academy f Sciences by the University f Gdansk Academic functins Head f Labratry f Mlecular Genetics f University f Gdansk 1996-present Head f Department f Mlecular Bilgy f University f Gdansk 1996-present Dean f Faculty f Bilgy, Gegraphy and Oceanlgy f University f Gdansk Vice-President fr Research Affairs f University f Gdansk 2008-present Academic staff educatin 37 PhDs prmted, 6 Assistant Prfessrs guided Membership and Functins in Prfessinal Organizatins Member f the Plish Bichemical Sciety 1991-present Member f Plish Genetic Sciety 1991-present, President f the Gdansk Branch , Member f the Main Cuncil , President Member f American Sciety fr Bichemistry and Mlecular Bilgy 2003-present Member f Internatinal Sciety fr Plasmid Bilgy 2003-present Expert experience (academic) Over 300 publicatins critically revieved in the internatinal peer-reviewed scientific jurnals as well as numerus Plish and freign grant applicatins reviewed. Editrial experience Editr f scientific jurnals FEMS Micrbilgy Reviews Plasmid Micrbial Cell Factries Acta Bichimica Plnica Editr-in-Chief 2009-present Member f Editrial Bards Jurnal f Applied Genetics Jurnal f Bimlecular Screening Metablic Brain Disease Oceanlgia Hnrs and Awards PhD thesis awarded by the President f the University f Gdansk 1992, Gdansk Award (schlarship) f the Fundatin fr Plish Science fr Outstanding Yung Scientists 1993, Warsaw Prizes f the Plish Bichemical Sciety fr the best experimental wrk in the field f bichemistry, perfrmed in Plish labratries and published in the frmer year 1994, 1995, 1999, 2000, 2004 Award f the Minister f Natinal Educatin fr scientific achievements (stringent DNA replicatin cntrl publicatin cycle) 1995 President f the University f Gdansk Award fr the Intercllegiate Faculty f Bitechnlgy UG-MUG management, develpment and rganizatin 1996, Gdansk Habilitatin awarded by the Prime Minister 1997, Warsaw Prizes f the Plish Genetic Sciety fr the best publicatins in the field f genetics, based n wrk perfrmed in Plish labratries 1999, 2001, 2004, 2007 Subsidy f the Fundatin fr Plish Science (prfessrship) 2000, Warsaw Plish Bichemical Sciety Award fr the best review article 2000, Warsaw President f the University f Gdansk Award, fr scientific achievements in the field f prtein stress prtectin prcess 2000, Gdansk

3 Medal f the Cmmittee fr Natinal Educatin 2001, Warsaw President f the University f Gdansk Award, fr scientific achievements in the field f the marine bacterium Vibri harveyi bilgy and its ptential use as an envirnmental mutagenic pllutin indicatr 2001, Gdansk President f the University f Gdansk Award, fr the tutrial achievements and the Faculty f Bilgy, Gegraphy and Oceanlgy management 2003, Gdansk President f the University f Gdansk Award, fr the rganisatin achievements 2004, Gdansk Hevelius Award Scientific Award f the City f Gdansk 2004, Gdansk Award f the Minister f Science and Higher Educatin fr scientific achievements 2010, Warsaw Scientific interests Plasmids and bacteriphage DNA replicatin mechanisms Cntrl f gene expressin in prkarytes Human genetic diseases, particularly neurdegenerative inherited metablic diseases Electrical bi-chips Scientific experience and achievements Reslving the replicatin mlecular mechanism f few plasmids with the stringent r relaxed metablic cntrl, pinpinting the essential rle f the transcriptin in the regulatin f DNA replicatin initiatin Mechanism f the DnaA prtein activated transcriptin, firstly claimed that bacterial transcriptinal activatrs can interact with the RNA plymerase beta subunit. Bacterial cells biluminescence plays a rle in DNA repair by stimulating the phtactivatin prcess. SeqA prtein, the main negative regulatr f the bacterial chrmsme replicatin initiatin, is als a transcriptin factr; describing its mechanism f actin. Describing the physilgical cnditins regulating the efficiency f the RNA pliadenylatin prcess in bacterial cells (as a first in the Wrld). Nvel treatment methd fr the lyssmal strage diseases, invlving flavnid genistein mdulated gene expressin. Prpsed bilgical functin f the Shiga-txin cding bacteriphages f the enterhemrrhagic Escherichia cli hst cells. Business experience and achievements Unitet States Patent, patent n. US 8,178,609 B2, 2012: Isflavnes fr treating mucplysaccharidses. Genzyme cmpany expert BiMarine cmpany expert Phage Cnsultants cmpany expert Cparatin with 3G Therapeutics cmpany in the field f research cmmercialisatin Scientific papers (Impact Factr 750, H-Index = 27, citatins - >3000, publicatins - >300, cnference cmmunicatins - >400, bks 1, edited bks 1) Patents 1. Unitet States Patent, patent n. US 8,178,609 B2, 2012, Isflavnes fr treating mucplysaccharidses ISI Master Jurnal List Publicatins (293 publicatins) 1. Obuchwski M, Węgrzyn G: Erichrme black T as a dye fr agarse gel electrphresis. Acta Bichimica Plnica 1991, 38: Węgrzyn G, Kwaśnik E, Taylr K: Replicatin f plasmid in amin acid-starved strains f Escherichia cli. Acta Bichimica Plnica 1991, 38: Grzegrz Węgrzyn, Neubauer P, Krueger S, Hecker M, Taylr K: Stringent cntrl f replicatin f plasmids derived frm cliphage. Mlecular and General Genetics 1991, 225: Węgrzyn G, Pawłwicz A, Taylr K: Stability f cliphage DNA replicatin initiatr, the O prtein. Jurnal f Mlecular Bilgy 1992, 226: Węgrzyn G, Taylr K: Inheritance f the replicatin cmplex by ne f tw daughter cpies during plasmid replicatin in Escherichia cli. Jurnal f Mlecular Bilgy 1992, 226:

4 6. Węgrzyn G, Glass RE, Thmas MS: Invlvement f the Escherichia cli RNA plymerase subunit in transcriptinal activatin by the bacteriphage lambda CI and CII prteins. Gene 1992, 122:1-7. Pawłwicz A, Węgrzyn G, Taylr K: Effect f cliphage P gene mutatins n the stability f the O prtein, the initiatr f DNA replicatin. Acta Bichimica Plnica 1993, 40: Salwa A, Węgrzyn G: Izlacja wirusa maedi wiec w Plsce. Medycyna Weterynaryjna 1993, 49: Herman A, Węgrzyn A, Węgrzyn G: Regulatin f replicatin f plasmid pbr322 in amin acidstarved Escherichia cli strains. Mlecular and General Genetics 1994, 243: Herman A, Węgrzyn A, Węgrzyn G: Cmbined effect f stringent r relaxed respnse, temperature and rm functin n the replicatin f puc plasmids in Escherichia cli. Acta Bichimica Plnica 1994, 41: Herman A, Węgrzyn A, Węgrzyn G: Differential replicatin f plasmids during stringent and relaxed respnse f Escherichia cli. Plasmid 1994, 32: Szalewska A, Węgrzyn G, Taylr K: Neither absence nr excess f O initiatr-digesting ClpXP prtease affects plasmid r phage replicatin in Escherichia cli. Mlecular Micrbilgy 1994, 13: Szalewska-Pałasz A, Węgrzyn A, Herman A, Węgrzyn G: The mechanism f the stringent cntrl f plasmid DNA replicatin. EMBO Jurnal 1994, 13: Szalewska-Pałasz A, Węgrzyn G: An additinal rle f transcriptinal activatin f ri in the regulatin f plasmid replicatin in Escherichia cli. Bichemical and Biphysical Research Cmmunicatins 1994, 205: Herman A, Węgrzyn G: Effect f increased ppgpp cncentratin n DNA replicatin f different replicns in Escherichia cli. Jurnal f Basic Micrbilgy 1995, 35: Taylr K, Węgrzyn G: Replicatin f cliphage lambda DNA. FEMS Micrbilgy Reviews 1995, 17: Węgrzyn G, Węgrzyn A, Knieczny I, Bielawski K, Knpa G, Obuchwski M, Helinski DR, Taylr K: Invlvement f the hst initiatr functin dnaa in the replicatin f cliphage. Genetics 1995, 139: Węgrzyn G, Szalewska-Pałasz A, Węgrzyn A, Obuchwski M, Taylr K: Transcriptinal activatin f cliphage DNA replicatin is regulated by the hst DnaA initiatr functin. Gene 1995, 154: Węgrzyn G: Czterfsfran guanzyny, ppgpp, jak czynnik ścisłej kntrli replikacji DNA. Pstępy Bichemii 1995, 41: Węgrzyn A, Węgrzyn G, Taylr K: Prtectin f cliphage O initiatr prtein frm prtelysis in the assembly f the replicatin cmplex in viv. Virlgy 1995, 207: Węgrzyn A, Węgrzyn G, Taylr K: Plasmid and hst functins required fr plasmid replicatin carried ut by the inherited replicatin cmplex. Mlecular and General Genetics 1995, 245:, Szalewska-Pałasz A, Węgrzyn G: Inhibitin f transcriptin starting frm bacteriphage pr prmter during the stringent respnse in Escherichia cli: implicatins fr DNA replicatin. Acta Bichimica Plnica 1995, 42: Węgrzyn G: Amplificatin f plasmids in Escherichia cli rela mutants. Jurnal f Bitechnlgy 1995, 43: Węgrzyn A, Węgrzyn G: Transcriptinal activatin f ri regulates plasmid replicatin in amin acid-starved Escherichia cli cells. Bichemical and Biphysical Research Cmmunicatins 1995, 214: Węgrzyn A, Węgrzyn G, Taylr K: Disassembly f the cliphage replicatin cmplex due t heat shck inductin f the gre pern. Virlgy 1996, 217: Neubauer P, Wróbel B, Węgrzyn G: DNA degradatin at elevated temperatures after plasmid amplificatin in amin acid-starved Escherichia cli cells. Bitechnlgy Letters 1996, 18: Obuchwski M, Węgrzyn G: Synthesis f the bacteriphage P prtein in amin acid-starved Escherichia cli cells. Bichemical and Biphysical Research Cmmunicatins 1996, 222: Węgrzyn G, Węgrzyn A, Pankiewicz A, Taylr K: Allele specificity f the Escherichia cli dnaa gene functin in the replicatin f plasmids derived frm phage. Mlecular and General Genetics 1996, 252: Węgrzyn A, Taylr K, Węgrzyn G: The cbpa chaperne gene functin cmpensates fr dnaj in plasmid replicatin during amin acid starvatin f Escherichia cli. Jurnal f Bacterilgy 1996, 178: Szalewska-Pałasz A, Węgrzyn A, Obuchwski M, Pawłwski R, Bielawski K, Thmas MS, Węgrzyn G: Drastically decreased transcriptin frm CII-activated prmters is respnsible fr impaired lysgenizatin f the Escherichia cli rpa341 mutant by bacteriphage. FEMS Micrbilgy Letters 1996, 144:21-27.

5 31. Węgrzyn A, Węgrzyn G, Herman A, Taylr K: Prtein inheritance: plasmid replicatin perpetuated by the heritable replicatin cmplex. Genes t Cells 1996, 1: Taylr K, Węgrzyn G, Węgrzyn A, Szalewska-Pałasz A, Herman A, Obuchwski M, Śrutkwska S, Knpa G: Escherichia cli initiatr prtein DnaA, cell cycle, and cntrl f plasmid replicatin. Bulletin f the Plish Academy f Sciences, Bilgical Sciences 1996, 44: Obuchwski M, Węgrzyn A, Szalewska-Pałasz A, Thmas MS, Węgrzyn G: An RNA plymerase subunit mutant impairs N-dependent transcriptinal antiterminatin in Escherichia cli. Mlecular Micrbilgy 1997, 23: Obuchwski M, Giladi H, Kby S, Szalewska-Pałasz A, Węgrzyn A, Oppenheim AB, Thmas MS, Węgrzyn G: Impaired lysgenisatin f the Escherichia cli rpa341 mutant by bacteriphage is due t the inability f CII t act as a transcriptinal activatr. Mlecular and General Genetics 1997, 254: Wróbel B, Węgrzyn G: Replicatin and amplificatin f plasmids in Escherichia cli during amin acid starvatin and limitatin. FEMS Micrbilgy Letters 1997, 153: Węgrzyn G: Antyterminacja transkrypcji u bakterifaga. Pstępy Bilgii Kmórki 1997, 24 (Suplement 8): Węgrzyn G, Węgrzyn A: Aktywacja transkrypcji w kmórkach Escherichia cli. Pstępy Bilgii Kmórki 1997, 24 (Suplement 8): Wróbel B, Węgrzyn G: Differential amplificatin efficiency f pmb1 and p15a (ClE1-type) replicns in Escherichia cli stringent and relaxed strains starved fr particular amin acids. Micrbilgical Research 1997, 152: Wróbel B, Węgrzyn G: Replicatin f plasmids derived frm P1, F, R1, R6K and RK2 replicns in amin acid-starved Escherichia cli stringent and relaxed strains. Jurnal f Basic Micrbilgy 1997, 37: Obuchwski M, Shtland Y, Kby S, Giladi H, Gabig M, Węgrzyn G, Oppenheim AB: Stability f CII is a key element in the cld stress respnse f bacteriphage infectin. Jurnal f Bacterilgy 1997, 179: Mikiewicz D, Wróbel B, Węgrzyn G, Płucienniczak A: Islatin and characterizatin f a ClE1-like plasmid frm Enterbacter agglmerans with a nvel variant f rm gene. Plasmid 1997, 38: Wróbel B, Węgrzyn G: Amplificatin f psc101 replicns in Escherichia cli during amin acid limitatin. Jurnal f Bitechnlgy 1997, 58: Taylr K, Węgrzyn G: Regulatin f bacteriphage replicatin. W: Mlecular Micrbilgy (S. J. W. Busby, C. M. Thmas, N. L. Brwn, Red.) 1998, Springer Verlag, Berlin-Heidelberg, str Wróbel B, Murphy H, Cashel M, Węgrzyn G: Guansine tetraphsphate (ppgpp)-mediated inhibitin f the activity f the bacteriphage pr prmter in Escherichia cli. Mlecular and General Genetics 1998, 257: Wróbel B, Węgrzyn G: Replicatin regulatin f ClE1-like plasmids in amin acid-starved Escherichia cli. Plasmid 1998, 39: Gabig M, Obuchwski M, Ciesielska A, Latała B, Węgrzyn A, Thmas MS, Węgrzyn G: The Escherichia cli RNA plymerase subunit and transcriptinal activatin by bacteriphage CII prtein. Acta Bichimica Plnica 1998, 45: Węgrzyn A, Herman-Antsiewicz A., Taylr K, Węgrzyn G: Mlecular mechanism f heat shckprvked disassembly f the cliphage replicatin cmplex. Jurnal f Bacterilgy 1998, 180: Szalewska-Pałasz A, Węgrzyn A, Błaszczak A, Taylr K, Węgrzyn G: DnaA-stimulated transcriptinal activatin f ri: Escherichia cli RNA plymerase subunit as a transcriptinal activatr cntact site. Prceedings f the Natinal Academy f Science f USA 1998, 95: Śrutkwska S, Knpa G, Węgrzyn G: A methd fr islatin f plasmid DNA replicatin intermediates frm unsynchrnized bacterial cultures fr electrn micrscpy analysis. Acta Bichimica Plnica 1998, 45: Wróbel B, Śrutkwska S, Węgrzyn G: Bichemical and genetic analysis f W, the newly islated lambdid phage. Acta Bichimica Plnica 1998, 45: Gabig M, Obuchwski M, Śrutkwska S, Węgrzyn G: Regulatin f replicatin f phage and plasmid at lw temperature. Mlecular and General Genetics 1998, 258: Wróbel B, Herman-Antsiewicz A, Szalewska-Pałasz A, Węgrzyn G: Plyadenylatin f p RNA in the regulatin f bacteriphage develpment. Gene 1998, 212: Herman-Antsiewicz A, Śrutkwska S, Taylr K, Węgrzyn G: Replicatin and maintenance f plasmids devid f the Cr repressr autregulatry lp in Escherichia cli. Plasmid 1998, 40: Węgrzyn A, Węgrzyn G: Randm inheritance f the replicatin cmplex by ne f tw daughter plasmid cpies after a replicatin rund in Escherichia cli. Bichemical and Biphysical Research Cmmunicatins 1998, 246:

6 55. Gabig M, Obuchwski M, Węgrzyn A, Szalewska-Pałasz A, Thmas MS, Węgrzyn G: Excess prductin f phage delayed early prteins under cnditins supprting high Escherichia cli grwth rates. Micrbilgy 1998, 144: Herman-Antsiewicz A, Węgrzyn G: Replicatin f plasmid DNA in the Escherichia cli cell cycle. Bichemical and Biphysical Research Cmmunicatins 1998, 247: Herman-Antsiewicz A, Węgrzyn A, Taylr K, Węgrzyn G: DnaA-mediated regulatin f phagederived replicns in the absence f pr and Cr functin. Virlgy 1998, 249: Szalewska-Pałasz A, Wróbel B, Węgrzyn G: Rapid degradatin f plyadenylated p RNA. FEBS Letters 1998, 432: Szalewska-Pałasz A, Weigel Ch, Speck Ch, Śrutkwska S, Knpa G, Lurz R, Marszałek J, Taylr K, Messer W, Węgrzyn G: Interactin f the Escherichia cli DnaA prtein with bacteriphage DNA. Mlecular and General Genetics 1998, 259: Szalewska-Pałasz A, Lemieszek E, Pankiewicz A, Węgrzyn A, Helinski DR, Węgrzyn G: Escherichia cli dnaa gene functin and bacteriphage replicatin. FEMS Micrbilgy Letters 1998, 167: Węgrzyn A, Szalewska-Pałasz A, Błaszczak A, Liberek K, Węgrzyn G: Differential inhibitin f transcriptin frm 70- and 32-dependent prmters by rifampicin. FEBS Letters 1998, 440: Ktlarz A, Szalewska-Pałasz A, Węgrzyn G, Lipińska B: Ecnmical and efficient verprductin f the Escherichia cli sigma 32 transcriptin factr. Bitechnlgy Techniques 1998, 12: Węgrzyn G: Replicatin f plasmids during bacterial respnse t amin acid starvatin. Plasmid 1999, 41: Węgrzyn A, Węgrzyn G: Regulacja replikacji DNA bakterifaga i plazmidów. Pstępy Bichemii 1999, 45: Glinkwska M, Węgrzyn A, Węgrzyn G: Replicatin f bacteriphage in the Escherichia cli dnaa rac hsts. Genetics 1999, 151: Śrutkwska S, Caspi R, Gabig M, Węgrzyn G: Detectin f DNA replicatin intermediates after tw-dimensinal agarse gel electrphresis using a flurescein-labeled prbe. Analytical Bichemistry 1999, 269: Węgrzyn A, Wróbel B, Węgrzyn G: Altered bilgical prperties f cell membranes in Escherichia cli dnaa and seqa mutants. Mlecular and General Genetics 1999, 261: Knpa G, Szalewska-Pałasz A, Schmidt A, Śrutkwska S, Messer W, Węgrzyn G: The presence f tw DnaA-binding sequences is required fr efficient interactin f the Escherichia cli DnaA prtein with each particular weak DnaA bx regin. FEMS Micrbilgy Letters 1999, 174: Kędzierska S, Staniszewska M, Ptrykus J, Węgrzyn G: The effect sme antibitic-resistancecnferring plasmids n the remval f the heat-aggregated prteins frm Escherichia cli. FEMS Micrbilgy Letters 1999, 176: Herman-Antsiewicz A, Węgrzyn G: Regulatin f cpy number and stability f phage derived ptc 1 plasmid in the light f the dimer/multimer catastrphe hypthesis. FEMS Micrbilgy Letters 1999, 176: Słmińska M, Neubauer P, Węgrzyn G: Regulatin f bacteriphage develpment by guansine 5'-diphsphate-3'-diphsphate. Virlgy 1999, 262: Messer W, Blaesing F, Majka J, Nardmann J, Schaper S, Schmidt A, Seitz H, Speck Ch, Tungler D, Węgrzyn G, Weigel Ch, Welzeck M, Zakrzewska-Czerwińska J: Functinal dmains f DnaA prteins. Bichimie 1999, 81: Szalewska-Pałasz A, Węgrzyn G: Czterfsfran guanzyny (ppgpp) - regulatr metablizmu kmórek bakteryjnych w warunkach głdwych. W: Na pgraniczu chemii i bilgii (H. Krniak, J. Barciszewski, Red.) 1999, Wydawnictw Nukwe Uniwersytetu im. Adama Mickiewicza w Pznaniu, tm III, str Czyż A, Wróbel B, Węgrzyn G: Vibri harveyi biluminescence plays a rle in stimulatin f DNA repair. Micrbilgy 2000, 146: Jakimwicz D, Majka J, Lis B, Knpa G, Węgrzyn G, Messer W, Schrempf H, Zakrzewska-Czerwińska J: Structure and regulatin f the dnaa prmter regin f three Streptmyces species. Mlecular and General Genetics 2000, 262: Czyż A, Jasiecki J, Bgdan A, Szpilewska H, Węgrzyn G: Genetically mdified Vibri harveyi strains as ptential biindicatrs f mutagenic pllutin f marine envirnments. Applied and Envirnmental Micrbilgy 2000, 66: Ptrykus K, Wróbel B, Węgrzyn A, Węgrzyn G: Replicatin f rij-based plasmid DNA during the stringent and relaxed respnses f Escherichia cli. Plasmid 2000, 44: Jakimwicz D, Majka J, Knpa G, Węgrzyn G, Messer W, Schrempf H, Zakrzewska-Czerwińska J: Architecture f the Sterptmyces lividans DnaA prtein-replicatin rigin cmplexes. Jurnal f Mlecular Bilgy 2000, 298: Knpa G, Barańska S, Węgrzyn A, Węgrzyn G: Bacteriphage and hst mutants causing the rlling-circle DNA replicatin early after infectin. FEBS Letters 2000, 472:

7 80. Węgrzyn A, Węgrzyn G: Frmatin and stability f bacteriphage replicatin cmplexes in UVirradiated Escherichia cli. Current Micrbilgy 2000, 41: Węgrzyn A, Czyż A, Gabig M, Węgrzyn G: ClpP/ClpX-mediated degradatin f the bacteriphage O prtein and regulatin f phage and plasmid replicatin. Archives f Micrbilgy 2000, 174: Guzw-Krzemińska A, Węgrzyn G: Ptential use f restrictin analysis f PCR-amplified DNA fragments in cnstructin f mlecular data-based identificatin keys f lichens. Myctaxn 2000, 76: Kijewska A, Słmińska M, Węgrzyn G, Rkicki J: A PCR-RFLP assay fr identificatin f Anisakis simplex frm different gegraphical regins. Mlecular and Cellular Prbes 2000, 14: Czyż A, Węgrzyn G: On the functin and evlutin f bacterial luminescence. W: Biluminescence and Chemiluminescence 2001(J. F. Case, P. J. Herring, B. H. Rbisn, S. H. D. Haddck, L. J. Kricka, P. E. Stanley, Red.), Wrld Scientific Publishing C., Singapre, str Latała B, Obuchwski M, Węgrzyn G: Bacteriphage ciii gene prduct has an additinal functin apart frm inhibitin f cii degradatin. Virus Genes 2001, 22: Czyż A, Zielke R, Knpa G, Węgrzyn G: Vibri harveyi insertinal mutant in the cgta (bg, yhbz) gene, whse hmlgues are present in diverse rganisms ranging frm bacteria t humans and are essential genes in many bacterial species. Micrbilgy 2001, 147: Barańska S, Gabig M, Węgrzyn A, Knpa G, Herman-Antsiewicz A, Hernandez P, Schvartzman JB, Helinski DR, Węgrzyn G: Regulatin f the switch frm early t late bacteriphage DNA replicatin mde. Micrbilgy 2001, 147: Węgrzyn A, Węgrzyn G: Inheritance f the replicatin cmplex: a unique r cmmn phenmenn in the cntrl f DNA replicatin? Archives f Micrbilgy 2001, 175: Herman-Antsiewicz A, Obuchwski M, Węgrzyn G: A plasmid clning vectr with precisely regulatable cpy number in Escherichia cli. Mlecular BitechnlgyC 2001, 17: Messer W, Blaesing F, Jakimwicz D, Majka J, Nardmann J, Schaper S, Seitz H, Speck Ch, Weigel Ch, Węgrzyn G, Welzeck M, Zakrzewska-Czerwińska J: Bacterial replicatin initiatr DnaA. Rules fr DnaA binding and rles f DnaA in rigin unwinding and helicase lading. Bichimie 2001, 83: Węgrzyn G, Węgrzyn A, Barańska S, Czyż A: Regulatin f bacteriphage lambda develpment. Recent Research Develpments in Virlgy 2001, 3, Jasiecki J, Czyż A, Gabig M, Węgrzyn G: Cnstructin and use f a brad-hst-range plasmid expressing the lamb gene fr utilizatin f bacteriphage vectrs in the marine bacterium Vibri harveyi. Marine Bitechnlgy 2001, 3: Wang Z, Le G, Shi Y, Węgrzyn G: Medium design fr plasmid DNA prductin based n stichimetric mdel. Prcess Bichemistry 2001, 36: Czyż A, Zielke R, Węgrzyn G: Rapid degradatin f bacteriphage O prtein by ClpP/ClpX prtease influences the lysis-versus-lysgenizatin decisin f the phage under certain grwth cnditins f the hst cells. Archives f Virlgy 2001, 146: Glinkwska M, Knpa G, Węgrzyn A, Herman-Antsiewicz A, Weigel Ch, Seitz H, Messer W, Węgrzyn G: The duble mechanism f incmpatibility between plasmids and Escherichia cli dnaa(ts) hst cells. Micrbilgy 2001, 147: Słmińska M, Węgrzyn A, Knpa G, Skarstad K, Węgrzyn G: SeqA, the Escherichia cli rigin sequestratin prtein, is als a specific transcriptin factr. Mlecular Micrbilgy 2001, 40: Czyż A, Łś M, Wróbel B, Węgrzyn G: Inhibitin f spntaneus inductin f lambdid prphages in Escherichia cli cultures: simple prcedures with pssible bitechnlgical applicatins. BMC Bitechnlgy 2001, 1: Węgrzyn G: The minimal genme paradx. Jurnal f Applied Genetics 2001, 42: Czyż A, Węgrzyn G: Mechanizm, regulacja i rla biluminescencji bakterii. Pstępy Bichemii 2001, 47: Gabig M, Węgrzyn G: An intrductin t DNA chips: principles, technlgy, applicatins and analysis. Acta Bichimica Plnica 2001, 48: Ptrykus J, Węgrzyn G: Chlramphenicl-sensitive Escherichia cli strain expressing the chlramphenicl acetyltransferase (cat) gene. Antimicrbial Agents and Chemtherapy 2001, 45: Guzw-Krzemińska B, Górniak M, Węgrzyn G: Mlecular determinatin keys: cnstructin f keys fr species identificatin based n restrictin fragment length plymrphism. Internatinal Archives f Biscience 2001, 1: Słmińska M, Knpa G, Węgrzyn G, Czyż A: Impaired chrmsme partitining and synchrnisatin f DNA replicatin initiatin in a Vibri harveyi insertinal mutant in the cgta gene cding fr a cmmn GTP-binding prtein. Bichemical Jurnal 2002, 362: Wróbel B, Wegrzyn G: Evlutin f lambdid replicatin mdules. Virus Genes 2002, 24:

8 105. Wang Z, Le G, Shi Y, Węgrzyn G, Wróbel B: A mdel fr regulatin f ClE1-like plasmid replicatin by uncharged trnas in amin acid starved Escherichia cli. Plasmid 2002, 47: Wang Z, Le G, Shi Y, Węgrzyn G: Purificatin f plasmid DNA using a multicmpartment electrlyser separated by ultrafilter membranes. Bitechnlgy Letters 2002, 24: Gabig M, Herman-Antsiewicz A, Kwiatkwska M, Łś M, Thmas MS, Węgrzyn G: The cell surface prtein Ag43 facilitates phage infectin f Escherichia cli in the presence f bile salts and carbhydrates. Micrbillgy 2002, 148: Barańska S, Knpa G, Węgrzyn G: Directinality f plasmid DNA replicatin carried ut by the heritable replicatin cmplex. Nucleic Acids Research 2002, 30: Ptrykus K, Perżył E, Węgrzyn G: po, a prmter fr p RNA synthesis, has a rle in replicatin f plasmids derived frm bacteriphage. Plasmid 2002, 47: Sikra-Brgula A, Słmińska M, Trznkwski P, Zielke R, Myśliwski A, Węgrzyn G, Czyż A: A rle fr the cmmn GTP-binding prtein in cupling f chrmsme replicatin t cell grwth and cell divisin. Bichemical and Biphysical Research Cmmunicatins 2002, 292: Ptrykus K, Barańska S, Węgrzyn A, Węgrzyn G: Cmpsitin f the plasmid heritable replicatin cmplex. Bichemical Jurnal 2002, 364: Czyż A, Plata K, Zielke R, Węgrzyn G: Luminescence-stimulated DNA repair in varius bacterial strains. W: Biluminescence and Chemiluminescence: Prgress and Current Applicatins (P. E. Stanley, L. J. Kricka, Red.) 2002, Wrld Scientific, Lndn, str Wang Z, Le G, Shi Y, Węgrzyn G: Studies n recvery plasmid DNA frm Escherichia cli by heat treatment. Prcess Bichemistry 2002, 38: Węgrzyn G, Thmas MS: Mdulatin f the susceptibility f intestinal bacteria t bacteriphages in respnse t Ag43 phase variatin a hypthesis. Medical Science Mnitr 2002, 8:HY Ptrykus J, Barańska S, Węgrzyn G: Inactivatin f the acra gene is partially respnsible fr chlramphenicl sensitivity f Escherichia cli CM2555 strain expressing the chlramphenicl acetyltransferase gene. Micrbial Drug Resistance 2002, 8: Czyż A, Plata K, Węgrzyn G: Inductin f light emissin by luminescent bacteria treated with UV light and chemical mutagens. Jurnal f Applied Genetics 2002, 43: Czyż A, Szpilewska H, Dutkiewicz R, Kwalska W, Biniewska-Gdlewska A, Węgrzyn G: Cmparisn f the Ames test and a newly develped assay fr detectin f mutagenic pllutin f marine envirnments. Mutatin Research 2002, 519: Węgrzyn G, Węgrzyn A: Stress respnses and replicatin f plasmids in bacterial cells. Micrbial Cell Factries 2002, 1: Ptrykus K, Sants S, Lemnnier M, Diaz-Orejas R, Węgrzyn G: Differential effects f Kid txin n tw mdes f replicatin f lambdid plasmids suggest that this txin acts befre, but nt after, the assembly f the replicatin cmplex. Micrbilgy 2002, 148: Dutkiewicz R, Słmińska M, Węgrzyn G, Czyż A: Overexpressin f the cgta (yhbz, bge) gene, cding fr an essential GTP-binding prtein, impairs the regulatin f chrmsmal functins in Escherichia cli. Current Micrbilgy 2002, 45: Czyż A, Łś M, Wróbel B, Węgrzyn G: Alleviatin f prblems with lambdid prphage inductin in Escherichia cli cultures. W: Life Sciences Technlgy 2002, Wrld Markets Research Centre, Lndn, str Węgrzyn G, Węgrzyn A: Regulatin f bacteriphage DNA replicatin. Current Tpics in Virlgy 2002, 2: Kijewska A, Rkicki J, Sitk J, Węgrzyn G: Ascarididea: a simple DNA assay fr identificatin f eleven species infecting marine and freshwater fish, mammals and fish-eating birds. Experimental Parasitlgy 2002, 101: Węgrzyn G, Czyż A: Hw d marine bacteria prduce light, why are they luminescent, and can we emply bacterial biluminescence in aquatic bitechnlgy? Oceanlgia 2002, 44: Ptrykus K, Węgrzyn G, Hernandez VJ: Multiple mechanisms f transcriptin inhibitin by ppgpp at the lambda PR prmter. Jurnal f Bilgical Chemistry 2002, 277: Węgrzyn G: Intrductin plasmids [Wstęp plazmidy]. Ksms 2002, 51: Czyż A, Węgrzyn G: Nwa metda wykrywania mutagennych zanieczyszczeń wdy mrskiej. Annales Academiae Medici Gedanensis 2002, 32: Guzw-Krzemińska B, Węgrzyn G: A preliminary study n the phylgeny f the genus Melanelia using nuclear large subunit ribsmal DNA sequences. Lichenlgist 2003, 35: Czyż A, Kwalska W, Węgrzyn G: Vibri harveyi mutagenicity assay as a preliminary test fr detectin f mutagenic pllutin f marine water. Bulletin f Envirnmental Cntaminatin and Txiclgy 2003, 70: Czyż A, Plata K, Węgrzyn G: Stimulatin f DNA repair as an evlutinary drive fr bacterial luminescence. Luminescence 2003, 18: