Curriculum Vitae Pitr Skwrn, Ph.D., D.Sc., Prfessr Current affiliatin and fficial address Department f Mlecular Bitechnlgy, Faculty f Chemistry, University f Gdansk, ul. Wita Stwsza 63, 80-308 Gdańsk, Pland. Tel. +48 660153424 Email: pitr.skwrn@ug.edu.pl; pmars44@gmail.cm Educatin, degrees and academic titles Studies 1981-1986, Bilgy, specialty: Mlecular Bilgy; Faculty f Bilgy and Earth Sciences, University f Gdansk, Pland M.Sc. 1986, Bilgy, specialty: Mlecular Bilgy, Faculty f Bilgy and Earth Sciences, University f Gdansk, Pland Ph.D. 1992, Bilgical Sciences (Bilgy), specialty: Mlecular Bilgy; McArdle Labratry fr Cancer Research, University f Wiscnsin-Madisn, USA, (prmter prf. Waclaw Szybalski) and Dept. f Micrbilgy, Faculty f Bilgy, Gegraphy and Oceanlgy, University f Gdansk, Pland (intercllegiate) Habilitatin (D.Sc.) 2011, Bilgical Sciences (Bilgy), specialty: Mlecular Bitechnlgy (new genetic engineering technlgies), Faculty f Bilgy, University f Gdansk, Pland Prfessr (title) 2014, Faculty f Bilgy, University f Gdansk, Pland Freign scientific schlarships and residences Mc Ardle Labratry fr Cancer Research, Faculty f Onclgy, University f Wiscnsin-Madisn, Wiscnsin (USA) 1989-1991 Mlecular Bilgy Resurces Inc. (USA), bitechnlgy industry 1992-1994 CHIMERx (USA), bitechnlgy industry 1994-1998 Vivantis Technlgies Sdn Bhd (Malaysia), bitechnlgy industry 2005 (1 mnth) Revngen Crp. (Malaysia), bitechnlgy industry 2012 (1 mnth) Career/emplyment Research Assistant 1985-1992, Department f Micrbilgy, Faculty f Bilgy, Gegraphy and Oceanlgy, University f Gdansk, Pland Ph.D. Student (stipend) 1989-1991, Mc Ardle Labratry fr Cancer Research, Faculty f Onclgy, University f Wiscnsin-Madisn, USA
Lecturer/researcher (Adjunct) 1992-1993, Department f Micrbilgy, Faculty f Bilgy, Gegraphy and Oceanlgy, University f Gdansk, Pland Specialist fr Mlecular Clning and Prtein Expressin 1992-1994, Mlecular Bilgy Resurces Inc., Wiscnsin, USA, (mlecular bitechnlgy cmpany, R&D divisin) Head f Research Team fr Mlecular Clning and Prtein Expressin 1994-1998, CHIMERx, Wiscnsin, USA, (mlecular bitechnlgy cmpany, R&D divisin) Lecturer/researcher (Adjunct) 1998-2001, Department f Micrbilgy, Chemical Faculty, Gdansk University f Technlgy, Pland Directr fr R&D, Vice President, c-funder, c-wner 1998-2007, EURx Ltd., Pland-USA, (mlecular bitechnlgy cmpany) Head f Department f Mlecular Bitechnlgy 2007-present, Institute fr Envirnment and Human Health Prtectin, Faculty f Chemistry, University f Gdansk, Pland Head f the Science-Industry Cnsrtium: University f Gdansk - POCh S.A. 2009-present, Directr fr R&D, President, c-funder, c-wner 2011-present BiVentures Institute Ltd., Pland (recmbinant vaccines) C-funder, c-wner 2012-present, Pharmris Ltd., Pland (pharmacy) Cfunder, c-wner 2012- present, MedVentures Ltd. Pland (bimedical applicatins) Lecturer/scientist (Adjunct) 2007-2012, Faculty f Chemistry, University f Gdansk, Pland Assciate Prfessr /Head f Dept. 2012-present, Faculty f Chemistry, University f Gdansk, Pland Prfessr /Head f Dept. 2012-present, Faculty f Chemistry, University f Gdansk, Pland Academic functins Head (and funder) f Department f Mlecular Bitechnlgy 2007-present, Institute fr Envirnment and Human Health Prtectin, Faculty f Chemistry, University f Gdansk, Pland Academic staff educatin 3 Ph.D. prmted, prmting currently 8 Ph.D. students, numerus prmted and guided M.Sc. students. Expert (academic) and editrial experience Expert fr the Natinal Centre fr Research and Develpment (NCBiR) (Pland) Expert fr the Plish Agency fr Enterprise Develpment (PARP) (Pland) Editr f grant applicatins, M.Sc. thesis, bks, internatinal publicatins frm ISI Master Jurnal List Hnrs and Awards Chemistry Olimpics Finalist (twice) 1978-1979 Champinship in academic pwerlifting/bdybuilding cmpetitin - 1982 MSc awarded by the President f University f Gdansk 1986 President f the University f Gdansk Award fr scientific achievements 1991 PhD thesis awarded by the President f University f Gdansk 1992 Mlecular Bilgy Resurces Inc. Awards (USA), fr industrial research and applicatins in the field f mlecular bitechnlgy 1994-2002 Interview in The Newsweek 2003 Brwn belt in Okinawa Gju-Ryu Karate D - 2005 Prmter f the MSc thesis, awarded 1st Prize by the Plish Chemical Sciety 2011 Prmter f the PhD thesis, distinguished by the Plish Chemical Sciety 2012 President f the University f Gdansk Award fr scientific achievements 2012 President f the University f Gdansk Award fr scientific achievements 2014 President f the University f Gdansk Award fr scientific achievements 2016 Pmeranian Emplyees Organizatin Primum Cperati award fr utstanding achievements in 25- years f academia-industry cperatin - 2015 Scientific interests New genetic engineering technlgies Restrictin endnucleases and ther DNA interacting prteins Recmbinant vaccine and engineered effectr prteins-based antiviral and anticancer therapies
Investigating and bitechnlgical explratin and prtectin f the bidiversity Scientific experience and achievements Clning, expressin and prtein engineering f ver 100 genes and their variants f an scientific, industrial and applicatins. Discvery and defining the new grup f enzymes cleaving DNA- bifunctinal restrictin endnucleasesmethyltransferases Thermus sp. Family, clning and characteristics f their cding genes, prteins and mechanism f actin. Inventr f nvel technlgy fr clning f extremely txic genes. Cnstructin f the expressin system based n the new psitive recmbinant selectin mechanism. C-authr f the cncept and experimental implementatin f tw DNA amplificatin technlgies, alternative t PCR. Develpment f DNA randmized fragmentatin technlgies fr genmic libraries cnstructins, DNA labeling and metagenmic research, with the use f artificial/mdified endnucleases. Develpment f methdlgy fr the labile DNA-restrictin endnuclease cmplexes investigatins. Develpment f a new generatin vaccine cnstructin technlgical platfrm Clning, expressin and applicatin f enzymes fr the use as antidtum and bisensr cmpnents t reverse symptms and detects warfare chemical weapns and pesticides Applied research, business and business-related experience and achievements 13 years f emplyment in American bitechnlgical industry, 11 years as Directr/Grup Leader f the bitechnlgy industrial research (5 years in the USA) Expert fr Vivantis Technlgies Sdn Bhd / BSTM Grup Sdn Bhd (Malaysia) 2010-present Expert fr Revngen Crpratin Grup (Malaysia, Thailand, Australia, Singapre e.a.) 2011-present First wrld develpment f technlgy fr DNA islatin in the ppular spin-micrclumn frmat (glbal market appraching 1 bln USD) 1990 17 patents/applicatins in the field f new genetic engineering technlgies (WO, PL, USA, EU, Israel, Japan, China, India) 78 cmpleted and applied industrial bitechnlgical prjects C-funder, directr fr R&D (develped majrity f cmpany recmbinant prducts) f the American- Plish bitechnlgical cmpany EURx Ltd. (Pland) Receiving R&D financing f app. 4 mln USD frm the Mlecular Bilgy Resurces Inc. (USA) 1998-2007 Funding the Science-Industry Cnsrtium: University f Gdansk - POCh Ltd - 2009-present, receiving NCBR develpment grant n NR12-0070-06/2009 (2009-2013 fr civil-military applicatins fr develpment f enzymes against chemical weapns (1,85 mln PLN). Receiving grant fr the industrial research in the field f the new vaccine technlgical platfrm, under the Innvative Ecnmy Operating Prgramme 1.4, NCBiR (2012-2015): POIG.01.04.00-22-140/12, Jagiellnian Innvatin Center, SATUS Venture Capital (4.6 mln PLN) Receiving grant fr the industrial research in the field f the new biactive hydrgel dressings f the new generatin, under the Innvative Ecnmy Operating Prgramme 3.1, NCBR (2007-2013), with Nickel Technlgy Park Pznan, UDA-POIG.03.01.00-00-010/10-00. (200,000 EURO) Initiated and received as cnsrtium partner a grant STRATEGMED1/235077/9/NCBR/2014 (acrnym REGENNOVA) fr the applied research in the field f the new regenerative medicine strategies, under the NCBR STRATEGMED prgramme (2014-2017) (ttal cnsrtium funding 17.8 mln PLN) Received as cnsrtium partner a grant STRATEGMED3/306853/9/NCBR/2017 (acrnym TARGETTELO) fr the applied research in the field f the new anty-cancer therapies, under the NCBR STRATEGMED prgramme (2017-2020) (ttal cnsrtium funding 19.7 mln PLN) C-funder and c-wner f 10 bitechnlgy cmpanies Publicatin list (Impact Factr app. 155, Index H = 14) Patents (issued and in prcessing) (26) 1. Mucha P, Załuska I, Czupryn A, Mazuryk J, Beręsewicz M, Krupska O, Wiśniewska M, Kziński K, Skwrn P, Palczewska Grves M, Żylicz-Stachula A, Żebrwska J, Rdziewicz-Mtwidł S, Pitrwski A, Pikuła M, Sachadyn P, Janus Ł: Peptydy plirgd trzymane metdą chemiczną i bitechnlgiczną działaniu przeciwuszkdzeniwym i/lub neurprtekcyjnym raz zestaw d znaczania ich aktywnści. (Eng.
2. Mucha P, Załuska I, Czupryn A, Mazuryk J, Beręsewicz M, Krupska O, Wiśniewska M, Kziński K, Skwrn P, Palczewska Grves M, Żylicz-Stachula A, Żebrwska J, Rdziewicz-Mtwidł S, Pitrwski A, Pikuła M, Sachadyn P, Janus Ł: PlyRGD peptides btained by chemical and bitechnlgy methds with injury-reducing and/r neurprtective effects and a kit fr their activity evaluatin. Eurpean Patent Office n EP 18000319.6 (2018). 3. Sachadyn P, Ssnwski P, Sass P, Pdlak-Ppinigis J, Górnikiewicz B, Czupryn A, Janus Ł, Mucha P, Pikuła M, Pitrwski A, Skwrn P, Rdziewicz-Mtwidł S: Kmpzycja farmaceutyczna zawierająca zebularynę raz śrdek leczniczy d regeneracji ran. (Eng. Use f xebularine fr prmting wund healing and regeneratin.) Republic f Pland Patent Office n P423672 (2018). 4. Sachadyn P, Ssnwski P, Sass P, Pdlak-Ppinigis J, Górnikiewicz B, Czupryn A, Janus Ł, Mucha P, Pikuła M, Pitrwski A, Skwrn P, Rdziewicz-Mtwidł S: Use f xebularine fr prmting wund healing and regeneratin. WO Patent Applicatin PCT/PL2018/000027 (2018). 5. Sachadyn P, Ssnwski P, Sass P, Pdlak-Ppinigis J, Górnikiewicz B, Czupryn A, Janus Ł, Mucha P, Pikuła M, Pitrwski A, Skwrn P, Rdziewicz-Mtwidł S: Use f epigenetic inhibitr 1-(β-D-ribfuransyl)-2(1H)-pyrimidinne fr prmting wund healing, cmplex tissue and rgan regeneratin. Eurpean Patent Office n EP18000264.4 (2018). 6. Rdziewicz-Mtwidł S, Pikuła M, Deptuła M, Karpwicz P, Sas P, Wardwska A, Sawicka J, Dzierżyńska M, Kasprzykwski F, Ssnwski P, Mieczkwska A, Filipwicz N, Madanecki P, Pitrwski A, Czupryn A, Mucha, P, Skwrn, P, Janus, Ł, Sachadyn P: Nwe peptydwe pchdne płytkpchdneg czynnika wzrstu (PDGF), spsób ich trzymywania, kmpzycja farmaceutyczna raz zastswanie. (Eng. New peptide derivatives f platelet derived grwth factr (PDGF), the methd f their preparatin, pharmaceutical cmpsitin and applicatin.) Republic f Pland Patent Office n P.425038 (2018). 7. Rdziewicz-Mtwidł S, Pikuła M, Deptuła M, Karpwicz P, Sas P, Wardwska A, Sawicka J, Dzierżyńska M, Kasprzykwski F, Ssnwski P, Mieczkwska A, Filipwicz N, Madanecki P, Pitrwski A, Czupryn A, Mucha, P, Skwrn, P, Janus, Ł, Sachadyn P: New peptide derivatives f platelet derived grwth factr (PDGF), the methd f their preparatin, pharmaceutical cmpsitin and applicatin. Eurpean Patent Office n EP18000305.5 (2018). 8. Skwrn PM, Brdzik RW, Kller K-P: Neutral heat-sensitive serine prtease derived frm O. crvinae. Eurpean Patent Office n EP17185519.0 (2017) 9. Skwrn P, Zylicz-Stachula A, Zlnierkiewicz O, Skwrn M, Janus L, Jezewska-Frackwiak J, Szymanska A: Spsób uzyskiwania białka pliepitpweg raz wektr DNA d realizacji teg spsbu. (Eng.: A methd f btaining a plyepitpic prtein as well as a DNA vectr fr embdying this methd.) Republic f Pland Patent Office n 228341 (2018) 10. Skwrn P, Jasiecki J: Spsób uzyskiwania rekmbinantwej, bilgicznie aktywnej butyrylchlinesterazy kńskiej i jej pchdnych raz spsób uzyskiwania bilgicznie aktywnych chlinesteraz i butyrylchlinesteraz raz ich pchdnych w mikrrganizmie Leishmania tarentlae. (Eng.: A methd f btaining a recmbinant, bilgically active chlinesterases and butyrylchlinesterases and their derivatives in micrrganism Leishmania tarentlae.) Republic f Pland Patent Office n P.423116 (2017) 11. Skwrn P, Krpinski A, Zylicz-Stachula A, Janus L, Szemiak K, Skwrn M, Bukrejewska M, Zwara A, Łś J, Łś M: Spsób wykrzystania sekwencji nukletydwej i kdwanych Otwartych Ram Odczytu genmu termfilneg bakterifaga, infekująceg gatunki bakterii Bacillus (Gebacillus). (Eng.: A methd f usage f a nucletide sequence and cded Open Reading Frames present in the termphilic bacteriphage, infecting species f Bacillus (Gebacillus) bacteria.) Republic f Pland Patent Office n P.418712 (2016) 12. Serczynska K, Bystrzycki Rman, Banaszczyk J, Wzniak D, Jędrzejczak G, Partryka T, Skwrn P, Jeżewska-Frąckwiak J: Samrzpuszczalna kapsułka d trzymywania rztwrów d zmywania. (Eng.: Self-sluble capsule fr the preparatin f washing slutins.) Republic f Pland Patent Office n P.419386 (2016) 13. Serczynska K, Bystrzycki Rman, Banaszczyk J, Wzniak D, Jędrzejczak G, Partryka T, Skwrn P, Jeżewska-Frąckwiak J: Self-sluble capsule fr the preparatin f washing slutins. Eurpean Patent Office n EP16207662.4 (2016) 14. Skwrn P, Zylicz-Stachula A, Zlnierkiewicz O, Skwrn M, Janus L, Jezewska-Frackwiak J, Krefft D, Nidzwrski D, Szemiak K, Maciejewska N, Nwak M, Szymanska A: A methd f btaining a plyepitpic prtein as well as a DNA vectr fr embdying this methd.
WO Patent Applicatin 2015162560 A1 (2015). In 2016 mdified and filed in: (15.) EU n EP15738474.4, (16.) USA n 15305453, (17.) China n CN 106488983 A, (18.) India n 201647039411, (19.) Israel n 248011, (20.) Japan n 2017-507091 21. Skwrn P, Jeżewska-Frąckwiak J, Serczynska K, Banaszczyk J, Wzniak D, Mazur P, Krefft D, Tadeusz Osswski T: Transfrmwana kmórka Escherichia cli raz spsób ceny skutecznści preparatów lub technik myjących w zmywaniu pwierzchni. (Eng.: A transfrmed Escherichia cli cell and a methd f evaluatin f an effectiveness f cleaning agents and surface cleaning techniques.) Republic f Pland Patent Office n P.415436 (2015) 22. Mead D, Skwrn PM, Swaminathan N, Van Etten J: Dinucletide restrictin endnuclease preparatins and methds f use. Eurpean Patent EP 0690870 A1 (1996) 23. Nickersn D, Mueller R, Skwrn P, Swaminathan N, Piehl R: DNA encding a thermstable DNA plymerase enzyme. WO Patent Applicatin 1,996,014,417 (1996) 24. Mueller R, Skwrn PM, Swaminathan N, Piehl R: Bilgically active fragments f Thermus flavus DNA plymerase. WO Patent Applicatin 1,996,014,405 (1996) 25. Mead D, Swaminathan N, Van Etten J, Skwrn PM: Recmbinant CviJI restrictin endnuclease. United States Patent n US005472872A, USA (1995) 26. Mead D, Swaminathan N, Van Etten J, Skwrn PM: Dinucletide restrictin endnuclease preparatins and methds f use. WO Patent Applicatin 1,994,021,663 (1994) ISI Master Jurnal List Publicatins (42 publicatins, IF app. 155, Index H = 14) 1. Krefft D, Papkv A, Prusinwski M, Skwrn PM, Zylicz-Stachula A: Randmized DNA libraries cnstructin tl: a new 3-bp frequent cutter TthHB27I/sinefungin endnuclease with chemically-induced specificity. BMC Genmics 2018, https://di.rg/10.1186/s12864-018-4748-0. 2. Skwrn PM, Krpinski AM, Zebrwska J, Janus L, Szemiak K, Czajkwska E, Maciejewska N, Skwrn M, Łś J, Łś M, Zylicz-Stachula A: Sequence, genme rganizatin, anntatin and prtemics f the thermphilic, 47.7-kb Gebacillus stearthermphilus bacteriphage TP-84 and its classificatin in the new Tp84virus genus. PLOS ONE 2018, 13: e0195449. 3. Jezewska-Frackwiak J, Serczynska K, Banaszczyk J, Wzniak D, Ozg A, Zylicz-Stachula A, Osswski T and Skwrn PM: Detectin f endspre prducing Bacillus species frm cmmercial prbitics and their preliminary micrbilgical characterizatin. J. Envirn. Bil. 2017, 38: 1435-1440. 4. Krefft D, Papkv A, Zylicz-Stachula A, Skwrn PM: Thermstable prteins biprcesses: The activity f restrictin endnuclease-methyltransferase frm Thermus thermphilus (RM.TthHB27I) clned in Escherichia cl is critically affected by the cdn cmpsitin f the synthetic gene. PLOS ONE 2017, 12:e0186633. 5. Skwrn PM, Antn BP, Czajkwska E, Zebrwska J, Sulecka E, Krefft D, Jezewska-Frackwiak J, Zlnierkiewicz O, Witkwska M, Mrgan RD, Wilsn GG, Fmenkv A, Rberts RJ and Zylicz- Stachula A: The third restrictin mdificatin system frm Thermus aquaticus YT-1: slving the riddle f tw TaqII specificities. Nucleic Acids Research 2017, di: 10.1093/nar/gkx599. 6. Zylicz-Stachula A, Zebrwska J, Czajkwska E, Wrese W, Sulecka E, Skwrn PM: Engineering TaqII bifunctinal endnuclease DNA recgnitin fidelity: the effect f a single amin acid substitutin within the methyltransferase catalytic site. Mlecular Bilgy Reprts 2016, 43:269-282. 7. Zebrwska J, Złnierkiewicza O, Skwrn MA, Zylicz-Stachula A, Jezewska-Frackwiak J, Skwrn PM: A putative Type IIS restrictin endnuclease GeICI frm Gebacillus sp - A rbust, thermstable alternative t mezphilic prttype BbvI. Jurnal f Bisciences 2016, 41:27-38. 8. Skwrn MA, 2, Zebrwska J, Wegrzyn G, Skwrn PM: MmSTI restrictin endnuclease, islated frm Mrganella mrgani infecting a trpical mth, Actias selene, cleaving 5 - CCNGG-3 sequences. J Appl Genetics 2016, 57:143-149. 9. Krefft D, Zylicz-Stachula A, Mulkiewicz E, Papkv A, Jezewska-Frackwiak J, Skwrn PM: Twstage gene assembly/clning f TthHB27I, a member f a TspDTI-subfamily f bifunctinal restrictin endnucleases. Jurnal f Bitechnlgy 2015, 194:67-80. 10. Jezewska-Frackwiak J, Lubys A, Vitkute J, Zakareviciene l, Zebrwska J, Krefft D, Skwrn M,
Agnieszka Zylicz-Stachula A, Skwrn PM: A new prttype IIS/IIC/IIG endnucleasemethyltransferase TsI frm the thermphile Thermus sctductus, recgnizing 5'-TARCCA(N11/9)-3' sequences. Jurnal f Bitechnlgy 2015, 194:19-26. 11. Zylicz-Stachula A, Plska K, Skwrn PM, Rak J: Artificial Plasmid Labeled with 5-Brm- 2 -dexyuridine: A Universal Mlecular System fr Strand Break Detectin. ChemBiChem 2014, 15:1409-12. 12. Zylicz-Stachula A, Zlnierkiewicz O, Sliwinska K, Jezewska-Frackwiak J, Skwrn PM: Mdified 'ne amin acid-ne cdn' engineering f high GC cntent TaqII-cding gene frm thermphilic Thermus aquaticus results in radical expressin increase. Micrbial Cell Factries 2014, 13:7. 13. Zylicz-Stachula A, Jezewska-Frackwiak J, Skwrn PM: Cfactr analgue-induced chemical reactivatin f endnuclease activity in a DNA cleavage/methylatin deficient TspGWI N 473A variant in the NPPY mtif. Mlecular Bilgy Reprts 2014, 41:2313-23. 14. Orlikwska M, Szymańska A, Brek D, Otwinwski Z, Skwrn PM, Jankwska E.: Structural characterizatin f V57D and V57P mutants f human cystatin C, an amylidgenic prtein. Acta Crystallgr D Bil Crystallgr. 2013, 69:577-86. 15. Skwrn PM, Vitkute J, Ramanauskaite D, Mitkaite G, Jezewska-Frackwiak J, Zebrwska J, Zylicz-Stachula A, Lubys, A: Three-stage bichemical selectin: clning f prttype class IIS/IIC/IIG restrictin endnuclease-methyltransferase TsI frm the thermphile Thermus sctductus. BMC Mlecular Bilgy, 2013, 14:17. 16. Zylicz-Stachula A, Zlnierkiewicz O, Jasiecki J, Skwrn PM: A new genmic tl: ultrafrequently cleaving TaqII/sinefungin endnuclease with cmbined 2.9 bp recgnitin site, applied t the cnstructin f hrse DNA libraries. BMC Genmics 2013, 14:370. 17. Żylicz-Stachula A, Żłnierkiewicz O, Lubys A, Ramanauskaite D, Mitkaite G, Bujnicki JM, Skwrn PM: Related bifunctinal restrictin endnuclease-methyltransferase triplets: TspDTI, Tth111II/TthHB27I and TsI with distinct specificities. BMC Mlecular Bilgy 2012, 13:13. 18. Żylicz-Stachula A, Żłnierkiewicz O, Śliwińska K, Jeżewska-Frąckwiak J, Skwrn PM: Bifunctinal TaqII restrictin endnuclease: redefining the prttype DNA recgnitin site and establishing the Fidelity Index fr partial cleaving. BMC Bichemistry 2011, 12:62. 19. Orlikwska M, Jankwska E, Brek D, Otwinwski Z, Skwrn P, Szymanska A: Crystallizatin and preliminary X-ray diffractin analysis f V57 mutants f an amylidgenic prtein - human cystatin C. Acta Crystallgraphica Sectin F 2011, F67:1608-1611. 20. Żylicz-Stachula A, Żłnierkiewicz O, Jeżewska-Frąckwiak J, Skwrn PM: Chemically-induced affinity star restrictin specificity: a nvel TspGWI/sinefungin endnuclease with theretical 3-bp cleavage frequency. BiTechniques 2011, 50:397-406. 21. Sblewski I, Plska K, Żylicz-Stachula A, Jeżewska-Frąckwiak J, Rak J, Skwrn PM: Enzymatic synthesis f lng duble-stranded DNA labeled with the halderivatives f nuclebases in a precisely pre-determined sequence. BMC Bichemistry 2011, 12:24. 22. Michalska B, Sblewski I, Plska K, Zielnka J, Żylicz-Stachula A, Skwrn PM, Rak J: PCR synthesis f duble stranded DNA labeled with 5-brmuridine. A step twards finding a brmnucleside fr clinical trials. Jurnal f Pharmaceutical and Bimedical Analysis 2011, 56:671-677. 23. Plska K, Zielnka J, Chmicz L, Czerwicka M, Stepnwski P, Guzw K, Wiczk W, Smużyńska M, Kasprzykwski F, Żylicz-Stachula A, Skwrn PM, Rak J: Unexpected phtprduct generated via the acetne- sensitized phtlysis f 5-Brm-2 -dexyuridine in a water/isprpanl slutin: experimental and cmputatinal studies. Jurnal f Physical Chemistry B 2010, 114: 16902-16907. 24. Żylicz-Stachula A, Bujnicki JM, Skwrn PM: Clning and analysis f bifunctinal DNA methyltransferase/nuclease TspGWI, the prttype f a Thermus sp. family. BMC Mlecular Bilgy 2009, 10:52. 25. Skwrn PM, Majewski J, Żylicz-Stachula A, Rutkwska SR, Jawrwska I, Harasimwicz- Słwińska R: A new Thermus sp. class-iis enzymes subfamily: islatin f a "twin" restrictin endnuclease TspDTI, with a nvel specificity 5`-ATGAA(N11/9)-3` related t TspGWI, TaqII and Tth111II. Nucleic Acid Research 2003, 31:e74. 26. Żylicz-Stachula A, Harasimwicz-Słwińska R, Sblewski I, Skwrn PM: TspGWI, a thermphilic class-iis restrictin endnuclease frm Thermus sp. recgnizes nvel asymmetric sequence 5`-ACGGA(N11/9)-3`. Nucleic Acid Research 2002, 30:e33. 27. Rutkwska SM, Skwrn PM: Rapid screening f elutin cnditins prir t immunpurificatin f prteins. BiTechniques 1999, 27:929-932. 28. Kaczrwski T, Sęktas M, Skwrn PM, Pdhajska AJ: The FkI methyltransferase frm
Flavbacterium keankites. Purificatin and characterizatin f the enzyme and its truncated derivatives. Mlecular Bitechnlgy (currently: New Bitechnlgy) 1999, 13:1-15. 29. Swaminathan N, McMaster K, Skwrn PM, Mead DA: Thermal cycle labeling: zeptmle detectin sensitivity and micrgram prbe amplificatin using CviJI* restrictingenerated lignucletides. Analytical Bichemistry 1998, 255:133-141. 30. Swaminathan N, Mead D, McMaster K, Gerge D, Van Etten JL, Skwrn PM: Mlecular clning f the three base restrictin endnuclease R.CviJI frm eukarytic Chlrella virus IL-3A. Nucleic Acid Research 1996, 24:2463-2469. 31. Skwrn PM, Harasimwicz R, Rutkwska SM: GCN4 eucarytic transcriptin factr/fki endnuclease mediated `Achilles Heel Cleavage`: quantitative study f prtein-dna interactin. Gene 1996, 170:1-8. 32. Lee JH, Skwrn PM, Rutkwska SM, Hng SS, Kim SC: Sequential amplificatin f clned DNA as tandem multimers using class-iis restrictin endnucleases. Genetic Analysis: Bimlecular Engineering 1996, 13:139-145. 33. Kim SC, Skwrn PM, Szybalski W: Structural requirements fr FkI-DNA interactin and ligdexyribnucletide-instructed cleavage. J. Mlecular Bilgy 1996, 258:638-649. 34. Rutkwska SM, Skwrn PM, Bielawski K, Pdhajska A: SacNI, a nvel BanII isschizmer frm Streptmyces achrmgenes recgnizing 5`-GRGCY/C-3`. Gene 1995, 157:319-320. 35. Rutkwska SM, Skwrn PM, Pdhajska A: Purificatin and characterizatin f the restrictin endnuclease AvcI frm Actinmyces cristalmycini. Gene 1995, 157:317-318. 36. Tuchlski J, Skwrn PM, Pdhajska A: MmeI, a class-iis restrictin endnuclease: purificatin and prperties. Gene 1995, 157:87-92. 37. Skwrn PM, Swaminathan N, McMaster K, Gerge D, Van Etten J, Mead D: Clning and applicatin f the tw/three-base restrictin endnuclease R.CviJI frm IL-3A virusinfected Chlrella. Gene 1995, 157:37-41. 38. Skwrn PM, Kaczrwski T, Tuchlski J, Pdhajska A: Atypical DNA-binding prperties f class-iis restrictin endnucleases: evidence fr recgnitin f the cgnate sequence by a FkI mnmer. Gene 1993, 125:1-10. 39. Fitzgerald MC, Skwrn PM, Van Etten JL, Smith LM, Mead DA: Rapid shtgun clning utilizing the tw base recgnitin endnuclease CviJI. Nucleic Acid Res. 1992, 20:3753-3762. 40. Piechula S, Skwrn PM, Piatyszek M, Pdhajska A: Islatin and identificatin f tw new Synechcccus-derived restrictin endnucleases, SleI and SspAI, isschizmers f EcRII. Nucleic Acid Res. 1991, 19:2782. 41. Kaczrwski T, Skwrn PM, Pdhajska AJ: Purificatin and characterizatin f the FkI Restrictin endnuclease. Gene 1989, 80:209-216. 42. Bielawski K, Skwrn PM, Kur J, Knpa G, Pdhajska A, Taylr K: Cnstructin f a DNAplymerase I verprducing plasmid and islatin f the enzyme. Acta Bichimica Plnica 1987, 34:29-34.