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A N N A L E S U N I V E R S I T A T I S M A R I A E C U R I E - S K Ł O D O W S K A L U B L I N P O L O N I A VOL. XVII (1) SECTIO EEE 27 Katedra Entomologii Akademii Rolniczej w Lublinie, ul. S. Leszczyskiego 7, 2-69 Lublin, e-mail: katarzyna.kmiec@ar.lublin.pl KATARZYNA KMIE Constancy of occurrence of aphid community (Hemiptera, Aphididae) on roses in the urban conditions of Lublin Stało wystpowania zespołu mszyc (Hemiptera, Aphididae) zasiedlajcego róe w warunkach miejskich Lublina Summary. The purpose of the present studies was to determine the frequency of particular species from the aphid community colonizing roses of different utility groups (Rosa rugosa Thunb., Rosa multiflora Thunb., park rose cv. Grandhotel, cluster flowered roses) growing in the urban conditions of Lublin. The studies were conducted in the years 21 23. The highest constancy of occurrence was characteristic of M. rosae. This species was observed annually on all roses in each site. The lowest frequency was characteristic of L. trirhodus, E. tiliae, M. euphorbiae, Aulacorthum sp. and those aphids belonged to accidents. Establishing the total frequency of the studied aphid species gives a picture of the occurrence of these species in a definite area. However, only the analysis of the structure of occurrence constancy of these species on definite host plants in particular sites makes it possible to grasp their interrelations. Key words: aphids, constancy of occurrence, frequency, roses INTRODUCTION With the aim of acquiring information on the commonness or rarity of a given species, its frequency or, in other words, constancy of occurrence is established. Frequency is multi-dimensional and it can inform about various aspects of species distribution. Constancy of occurrence is a narrower range of frequency, and its type is analyzed on the basis of estimation of frequency [Górny and Grm 1981, Czachorowski 24]. The purpose of the present studies was to determine the frequency of particular species from the aphid community colonizing roses of different utility groups growing in the urban conditions of Lublin.

54 K. Kmie MATERIAL AND METHODS The studies were conducted in the years 21 23 in four sites of the Lublin green areas: a road site (A), a street site (B), a housing estate site (C) and a park site (D). In each of the sites fifteen shoots of similar length were analyzed on the following roses: a park variety Grandhotel, a cluster flowered rose of different varieties, a saltspray rose (Rosa rugosa Thunb.) and a multiflowered rose (Rosa multiflora Thunb.). In each season 28 samples were collected, from early spring (second 1-days period of March) till late autumn (second 1-days period of December) with about 1-days intervals. The analysis of the obtained data was conducted on the basis of papers by Górny and Grm [1981] and Czachorowski [24]. In order to determine the constancy of occurrence of particular aphid species, index (C) was used, which expressed the relation of the number of samples where a given species occurred to the number of all samples: q C = 1% Q where: q number of trials when a given species was noted, Q number of all samples. Five classes of frequency were distinguished: I. euconstants 71.% II. constants 46. 7.9% III. subconstants 26. 45.9% IV. accessory species 11. 25.9% V. accidents 1.9% The present paper treats a single observation as a sample. Calculating the total frequency of aphids, number of all observations (3 seasons 28 observations = 84) during three seasons was taken as value Q, while q was the number of observations where a given species was noted during three seasons. Calculating the constancy of occurrence of the observed aphid species on particular roses, Q value was like above, whereas q was the number of observations in which a given species was observed on a specific species or variety of rose. Besides, the constancy of occurrence of the studied aphid species was established on the analyzed roses in particular sites, where Q value was like above, while q was the number of observations where a given aphid species was noted on a definite species or variety of rose in a given site. RESULTS The observations performed in the years 21 23 on roses of selected utility groups in the urban conditions of Lublin found out the occurrence of 1 aphid species (Hemiptera, Aphididae): Macrosiphum rosae (L.), Chaetosiphon tetrarhodus (Walk.), Metopolophium dirhodum (Walk.), Myzaphis rosarum (Kalt.), Maculolachnus submacula (Walk.), Longicaudus trirhodus (Walk.), Aphis fabae Scop., Macrosiphum euphorbiae (Thom.), Eucalipterus tiliae (L.), Aulacorthum sp.

CONSTANCY OF OCCURRENCE OF APHID COMMUNITY... 55 Total aphid frequency in the area of Lublin The acquired results made it possible to classify the observed aphid species to 5 frequency classes (Tab. 1). The class of euconstants was made of M. rosae. It was the species which was observed annually on all roses in each site, while during the vegetation season it occurred on plants most often. The class of constants included Ch. tetrarhodus and M. submacula. The subconstants also included two species: M. dirhodum and M. rosarum. The accessory species was A. fabae. The class of accidents was made up of four species: L. trirhodus, E. tiliae, M. euphorbiae and Aulacorthum sp. Table 1. Total aphid frequency in the area of Lublin in the years 21 23 Tabela 1. Ogólna frekwencja mszyc na terenie Lublina w latach 21 23 Class of frequency Klasa frekwencji Euconstants Eukonstanty Constants Konstanty Subconstants Subkonstanty Accessory species Gatunki akcesoryczne Accidents Akcydenty Species of aphid Index Gatunek mszyc 21 22 23 total Macrosiphum rosae 78.6 78.6 71.4 76.2 Chaetosiphon tetrarhodus Maculolachnus submacula Metopolophium dirhodum Myzaphis rosarum 71.4 78.6 57.1 35.7 64.3 64.3 42.8 6.7 75. 46.4 35.7 32.1 7.2 63.1 45.2 42.8 Aphis fabae 25. 17.8 17.8 2.2 Longicaudus trirhodus Eucalipterus tiliae Macrosiphum euphorbiae Aulacorthum sp. 14.3 7.1 17.8 1 17.8 14.3 3.6 7,1 1.7 9.5 7.1 1.2 The structure of occurrence constancy of aphid community on particular roses Analyzing the obtained results, significant differences were found out in the length of the period of the analyzed aphid species staying on a given rose. The most differentiated structure of occurrence constancy was marked on the park rose Grandhotel and saltspray rose, where four constancy classes were distinguished on each (no class of euconstants). The greatest differences in the content of particular classes were found in the case of M. rosae and Ch. tetrarhodus (Tab. 2). The first species on the park rose Grandhotel belonged to constants, while on the R. rugosa to subconstants. Chaetosiphon tetrarhodus, which on the saltspray rose constituted the class of constants, on the park rose Grandhotel belonged to accessory species with almost three times lower frequency as compared to the above mentioned rose. Relatively high frequency of M. submacula on the park rose made it possible to include it within subconstants. On the other hand, the other aphid species observed on those roses were included in the same classes of constancy. Only two constancy classes were isolated on multiflowered rose and cluster flowered roses. Macrosiphum rosae was included in accessory species, while the other aphid species were accidents.

Table 2. The structure of occurrence constancy (C) of aphid community on particular roses in the years 21 23 Tabela 2. Struktura stałoci wystpowania (C) zespołu mszyc na poszczególnych róach w latach 21 23 Class of frequency Klasa frekwencji Euconstants Eukonstanty Constants Eukonstanty Subconstants Subkonstanty Accessory species Gatunki akcesoryczne Accidents Akcydenty Saltspray rose Róa pomarszczona Park rose Grandhotel Róa parkowa Ch. tetrarhodus 57.1 M. rosae 48.8 M. rosae 31.2 M. submacula 27.4 M. dirhodum 24.4 Ch. tetrarhodus M. dirhodum M. rosarum L. trirhodus M. euphorbiae 2.1 1.2 L. trirhodus A. fabae M. euphorbiae E. tiliae. 19.9 22.7 13.4 5. 9.2 1.5 2.7 Multiflowered rose Róa wielokwiatowa Cluster flowered roses Róe rabatowe M. rosae 14.9 M. rosae 23.2 Ch. tetrarhodus M. dirhodum Aulacorthum sp. 9.5 3..3 Ch. tetrarhodus M. dirhodum M. rosarum M. submacula M. euphorbiae E. tiliae.9 5.6 5.3 9.5.6.6

CONSTANCY OF OCCURRENCE OF APHID COMMUNITY... 57 Structure of occurrence of aphid community on the analyzed roses in particular sites Analyzing occurrence constancy on the studied roses in particular sites presented in Table 3, preferences of those insects were very clearly found out towards the host plant (also seen in Table 2). On the other hand, the studies did not find any significant effect of the type of site on their constancy of occurrence with an exception of two aphid species. The most differentiated structure of constancy was characteristic of M. submacula on the park rose. In the street site, it belonged to constants, in the road site to subconstants, in the housing estate to accessory species, while in the park site it was not present on this rose. The species if M. rosarum on the park rose in the road site belonged to subconstants, while in the other sites it belonged to accidents. In the case of the other aphid species differences in the constancy of occurrence between particular sites were insignificant or not present at all. Table 3. Structure of occurrence constancy of aphid community on the analyzed roses in particular sites in the years 21 23 Tabela 3. Struktura stałoci wystpowania zespołu mszyc na analizowanych róach w poszczególnych stanowiskach w latach 21 23 Species of aphid Gatunek mszyc saltspray pomarszczona park Grandhotel parkowa Roses/Róe multiflowered wielokwiatowa cluster flowered rabatowa A B C D A B C D A B C D A B C D Ch. tetrarhodus II II II III IV IV IV IV IV V IV V V V - - M. rosae III III III III III III II II IV IV IV V III IV IV IV M. dirhodum IV III IV IV IV III IV IV V V - - V V V V M. rosarum - - - - III V V V - - - - V V V V M. submacula - - - - III II IV - - - - - IV V V V L. trirhodus V V V - V V V V - - - - - - - - A. fabae - - - - V IV - IV - - - - - - - - M. euphorbiae - V V - V V V - - - - - V - V - E. tiliae - - - - V V - - - - - - - V - - Aulacorthum - - - - - - - - - V - - - - - - Sites/Stanowiska: A road/przyjezdniowe, B street/przyuliczne, C housing estate/osiedlowe, D park/parkowe; no aphids/brak mszyc. Constancy of aphid appearance/stało wystpowania mszyc: Class I euconstants/eukonstanty 71.%; Class II constants/konstanty 46. 7.9%; Class III subconstants/subkonstanty 26. 45.9%; Class IV accessory species/gatunki akcesoryczne 11. 25.9%; Class V accidents/akcydenty 1.9% DISCUSSION The results discussed above testify to the multidimensional character of frequency and, at the same time, to the possibility of calculating it in reference to various levels, which is pointed to by Czachorowski [24].

58 K. Kmie Macrosiphum rosae is a common species on roses which usually remains on the shrubs throughout most of the vegetation period [Jakiewicz 23]. The species that occurred on all roses in most sites included Ch. tetrarhodus and M. dirhodum. The former was characterized by high total frequency; however, its frequency on particular roses was differentiated since this is a species with clearly marked preferences in relation to the host plant [Jakiewicz and Kmie 25]. Metopolophium dirhodum, although a host-alternating species, occurred relatively often on the shrubs. High total frequency was characteristic of M. submacula, but this is a species preying mostly on noble roses and it is closely associated with the specified site [Jakiewicz et al. 21, Jakiewicz and Kmie 24]. Relatively high total frequency was found for M. rosarum, but it was caused by its frequent occurrence on the park rose Grandhotel only in the road site. Aphis fabae were observed only on the park rose Grandhotel, where in summer it preyed at the base of the petals. Longicaudus trirhodus, enumerated by Cichocka and Jakiewicz [23] as belonging to the species associated with rose (Rosa L.) was observed fairly rarely and only on certain shrubs. Very low frequency was characteristic of E. tiliae, M. euphorbiae and Aulacorthum sp., which should be considered accidental species. The first one is connected to linden through its feeding, while the second is a polyphagous species and it is observed on roses in greater numbers mainly under covers [Wojdyła et al. 22]. On the other hand, aphid larvae of Aulacorthum genus were found only in one observation on one shrub of R. multiflora. Establishing the total frequency of the studied aphid species gives a picture of the occurrence of these species in a definite area in this case in the area of Lublin. However, only the analysis of the structure of occurrence constancy of these species on definite host plants in particular sites makes it possible to grasp their interrelations. CONCLUSIONS 1. The highest constancy of occurrence was characteristic of M. rosae. This species was observed annually on all roses in each site. 2. The lowest frequency was characteristic of L. trirhodus, E. tiliae, M. euphorbiae, Aulacorthum sp. and those aphids belonged to accidents. 3. Establishing the total frequency of the studied aphid species gives a picture of the occurrence of these species in a definite area. The analysis of the structure of occurrence constancy of these species on definite host plants in particular sites makes it possible to grasp their interrelations. REFERENCES Cichocka E., Jakiewicz B. 23. Aphid inhabiting roses in different cultures in 1967 23. Aphids and Other Hemipterous Insects, Monograph, PAG, Rogów, 9, 17 36. Czachorowski S. 24. Opisywanie biocenozy zoocenologia. Skrypt elektroniczny dla magistrantów. www.uwm.edu.pl/czachor/publik/pdf-inne/zoocenozy.pdf. Górny M., Grüm L. 1981. Metody stosowane w zoologii gleby. PWN, Warszawa, 397 416.

CONSTANCY OF OCCURRENCE OF APHID COMMUNITY... 59 Jakiewicz B., Kmie K. 24. Maculolachnus submacula (Walk.) wystpujca na róach na terenach zieleni miejskiej Lublina. [w:] P. Indykiewicz, T. Barczak (red.) Fauna miast Europy rodkowej 21. wieku. Wyd. LOGO, Bydgoszcz, 147 154. Jakiewicz B., Kmie K. 25. The Population Dynamics of Chaetosiphon tetrarhodus (Walk.) in Urban Conditions. Aphids and other Hemipterous Insects, Sieraków, 11, 63 73. Jakiewicz B., Kmie K., Bartoszek A. 21. The occurrence of Maculolachnus submacula Walk. on rose bushes Rosa canina L. under city conditions. [in:] Aphids and other Homopterous Insects, Polish Academy of Science, Siedlce, 8, 271 279. Wojdyła A., Kami ska M., Łabanowski G., Orlikowski L. 22. Ochrona ró. Plantpress, Kraków, 89. Streszczenie. Celem przeprowadzonych bada było ustalenie frekwencji poszczególnych gatunków z zespołu mszyc zasiedlajcego róe z rónych grup uytkowych (róa pomarszczona Rosa rugosa, róa wielokwiatowa Rosa multiflora, róa parkowa Grandhotel, róe rabatowe), rosnce w warunkach miejskich Lublina. Badania prowadzono w latach 21 23. Najwysz stało- ci wystpowania charakteryzował si gatunek M. rosae, który notowano corocznie na wszystkich badanych róach w kadym ze stanowisk. Najnisz frekwencj charakteryzowały si gatunki L. trirhodus, E. tiliae, M. euphorbiae, Aulacorthum sp. nalece do klasy akcydentów. Ustalenie ogólnej frekwencji badanych gatunków mszyc daje pewien obraz ich wystpowania na okrelonym obszarze. Jednak dopiero analiza struktury stałoci wystpowania tych gatunków na okrelonych rolinach ywicielskich w poszczególnych stanowiskach pozwala okreli ich wzajemne powizania. Słowa kluczowe: mszyce, stało wystpowania, frekwencja, róe