PRELIMINARY RESEARCH ON MESOSTIGMATA MITES (ACARI) FROM A SPRUCE FOREST IN THE BUCEGI MASSIF IN ROMANIA



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SCIENTIARUM POLONORUMACTA Silv. Colendar. Rat. Ind. Lignar. 3(2) 2004, 79-84 PRELIMINARY RESEARCH ON MESOSTIGMATA MITES (ACARI) FROM A SPRUCE FOREST IN THE BUCEGI MASSIF IN ROMANIA Minodora Stănescu 1, Dariusz J. Gwiazdowicz 2 1 Institute of Biology, Bucharest, 2 Agricultural University of Poznań Abstract. This communication presents preliminary results of studies on the mite fauna observed in the spruce stands of the Bucegi Massif in central Romania. In the collected material a total of 6436 specimens were found, which were classified to 62 species from 15 families. The most numerous were the species Veigaia nemorensis (1660 individuals), Leptogamasus parvulus (804) and Leptogamasus tectegynellus (694), whereas Veigaia nemorensis (224 samples), Neopodocinum mrciaki (150) and Pachyseius humeralis (150) were observed in the biggest number of samples. In the litter layer a total of 4842 specimens were found of mites belonging to 54 species, whereas in the humus layer it was 1594 specimens belonging to 47 species. The species similarity index between mite communities of the litter and humus layers was very high and amounts to over 77%. Key words: mites, Acari, Mesostigmata, litter, Bucegi Massif, Romania INTRODUCTION The Carpathian Mountains are one of the bigger mountains ranges in Europe, with considerable biological diversification. The differentiation in terms of altitude, rocks and soil, precipitation, as well as vegetation has resulted in the abundance and diversity of acarifauna. Studies on mites found in these mountains have been conducted for several years in Poland [Skorupski and Gwiazdowicz 1996, Gwiazdowicz and Sznajdrowski 2000, Gwiazdowicz and Cichocki 2002] and Slovakia [Fenda and Mašan 2003, Mašan and Fenda 2003). Unfortunately, the fauna of mites from order Mesostigmata found in the Romanian part of the Carpathian Mountains is relatively little known. Exceptions in this respect are studies concerning family Parasitidae conducted by Juvara-Bals [1974, 1976] and studies conducted in several Carpathian ranges by Honciuc and Stǎnescu [2003]. For Corresponding author Adres do korespondencji: Minodora Stănescu, Institute of Biology, Department of Ecology and Natural Protection, Splaiul Independenţei Street 296, 060031 Bucharest, Romania, e-mail: stanescumina@hotmail.com

80 M. Stănescu, D.J. Gwiazdowicz this reason in 2003 an international research project was initiated, the objective of which was to determine the diversity of acarifauna in the Carpathian Mountains in Romania and to compare the obtained results with those found in other countries. In this way it will be possible to determine distribution ranges of selected species and to define their site preferences, e.g. such as. This communication presents preliminary results of investigations on the acarifauna in spruce stands of the Bucegi Massif. METHODS AND RESEARCH AREA Field studies covered spruce stands of the Bucegi Massif in central Romania (N 45 23 587 ; E 025 31 959 ) and samples were collected in the years 2001-2003. A total of 336 samples were collected using the MacFadyen soil core. Each sample was divided into the litter (OLF) and humus (OH) layer. The extraction was performed with modified Berlese-Tullgren funnels. The Bucegi Massif is situated on the east of the Meridionali Carpathiens. The borders of this massif are: on east Prahova Valley, on west Dâmboviţa Valley, on north Braşov city and on south Curburii Subcarpathians. The Bucegi Massif has two parts in the north-southern direction, linked by the Omul peak, the highest in this part of the Carpathian Mts. (2507 m altitude). To the east there is a big rock wall named Abruptul Prahovean. The whole massif is crossed by numerous streams, affluents of the Prahova river. This zone is representative for the forest ecosystems with spruce Picea abies, with beech Fagus sylvatica and their mixture. From the geological point of view, this massif has a coarcal structure. The ecosystem with spruce is situated on a slope of 20, on the east side of the mountain. This forest has trees from 40 to 120 years old and the proportion of the cover is 85% and the consistence is 0.7. The herbaceous layer and the shrub layer are missing. The litter layer is continuous and thin (1-2 cm) and humus is of moder-mull type. RESULTS AND CONCLUSIONS On the basis of the collected material a total of 6436 specimens of mites of the order Mesostigmata were found belonging to 62 species (Table 1). The following species were most numerous in the collected material: Veigaia nemorensis (1660 specimens), Leptogamasus parvulus (804), Zercon triangularis (762), Leptogamasus tectegynellus (694). In turn, Veigaia nemorensis (224 samples), Neopodocinum mrciaki (150), Pachyseius humeralis (150), Leptogamasus parvulus (140) and Pergamasus athiasae (136) were observed in the biggest numbers of samples. In the litter layer OLF a total of 4842 mite specimens were found, belonging to 54 species. These species are eudominant in this layer: Veigaia nemorensis (26%), Zercon triangularis (14%), Leptogamasus parvulus (13%), Leptogamasus tectegynellus (11%) and another are dominant like: Neopodocinum mrciaki (8%), Zercon fageticola (8%) and Pachyseius humeralis (6%).

Preliminary research on Mesostigmata mites... 81 Table 1. Mesostigmatic mites recorded from the Norway spruce forest litter of Bucegi Massif in Romania Tabela 1. Roztocze z rzędu Mesostigmata wykazane w ściółce w drzewostanach świerkowych w Bucegi Massif w Rumunii Family, species Rodzina, gatunek Litter Ściółka Samples Próby Humus Humus 1 2 3 Zerconidae Polonozercon tatrensis Błaszak, 1974 2 4 Prozercon kochi Sellnick, 1943 50 22 Prozercon traegardhi Halbert, 1923 10 20 Prozercon sellnicki Halaskova, 1963 8 4 Zercon carpathicus Sellnicki, 1958 6 2 Zercon fageticola Halaškova, 1969 384 96 Zercon peltatus peltadoides Halaškova, 1970 16 6 Zercon pinicola Halaškova, 1970 2 Zercon romagniolus Sellnicki, 1944 4 Zercon similis Willmann, 1953 2 Zercon triangularis C.L. Koch, 1836 664 98 Parasitidae Eugamasus magnus Kramer, 1876 14 Parasitus furcatus G. and R. Canestrini, 1882 4 Porrhostaspis lunulata Müller, 1859 6 2 Holoparasitus calcaratus C.L. Koch, 1839 4 Vulgarogamasus cornutosimilis Schweizer, 1949 4 2 Vulgarogamasus kraepelini Berlese, 1905 36 8 Vulgarogamasus oudemansi Berlese, 1903 6 8 Vulgarogamasus zschokkei Schweizer, 1922 44 8 Holoparasitus excisus Berlese, 1905 2 Leptogamasus doinae Juvara-Balş, 1981 4 Leptogamasus parvulus Berlese, 1903 630 174 Leptogamasus tectegynellus Athias Henriot, 1967 524 170 Pergamasus alpinus Berlese, 1903 4 Pergamasus athiasae Juvara-Balş, 1970 38 14 Pergamasus laetus Juvara-Balş, 1970 22 28 Pergamasus quisquiliarum Canestrini, 1882 4 Macrochelidae Geholaspis longispinosus Kramer, 1876 10 4 Macrocheles montanus Willmann, 1951 14 6 Neopodocinum mrciaki Sellnick, 1968 362 54 Eviphididae Eviphis ostrinus C.L. Koch, 1836 36 10 Silvarum Colendarum Ratio et Industria Lignaria 3(2) 2004

82 M. Stănescu, D.J. Gwiazdowicz 1 2 3 Ascidae Arctoseius cetratus Sellnick, 1940 12 14 Arctoseius eremitus Berlese, 1918 10 4 Arctoseius magnanalis Evans, 1958 14 10 Arctoseius semiscissus Berlese, 1892 50 16 Arctoseius venustulus Berlese, 1917 2 Proctolaelaps juradeus Schweizer, 1949 6 4 Zerconopsis remiger Kramer, 1876 4 Laelapidae Hypoaspis aculeifer Canestrini, 1883 58 88 Veigaiaiidae Veigaia exigua Berlese, 1916 26 26 Veigaia cervus Kramer, 1876 14 4 Veigaia nemorensis C.L. Koch, 1839 1 266 394 Veigaia propinqua Willmann, 1936 18 Rhodacaridae Rhodacarellus kreuzi Karg, 1965 20 16 Pachylaelapidae Pachylaelaps furcifer Oudemans, 1903 12 6 Pachyseius humeralis Berlese, 1910 306 152 Digamasellidae Dendrolaelaps foveolatus Leitner, 1949 4 2 Dendrolaelaps rotundus Hirschmann, 1960 6 28 Ameroseiidae Epicriopsis rivus Karg, 1971 6 Trachytidae Trachytes aegrota C.L. Koch, 1841 60 14 Trachytes irenae Pecina, 1970 8 Trachytes pauperior Berlese, 1914 8 26 Trematuridae Trichouropoda calcarata Hirschmann et Zirngiebl-Nicol, 1961 6 Trichouropoda obscurasimilis Hirschmann et Zirngiebl-Nicol, 1961 14 Trichouropoda punctata Hirschmann et Zirngiebl-Nicol, 1961 8 Urodinychidae Dinychus carinatus Berlese, 1903 4 Dinychus perforatus Kramer, 1882 2 Urodiaspis pannonica Willmann, 1952 6 Urodiaspis tecta Kramer, 1876 10 4 Uroobovella obovata Canestrini et Berlese, 1884 2 4 Uroobovella pulchella Berlese, 1904 2 Uropodidae Uropoda splendida Kramer, 1882 4 Number of species 54 47 Number of specimens 4 842 1 594

Preliminary research on Mesostigmata mites... 83 In the humus layer OH a total of 1594 mite specimens were observed, belonging to 47 species. Veigaia nemorensis (17%) is an eudominant species, Leptogamasus parvulus (7%), Leptogamasus tectegynellus (7%) and Pachyseius humeralis (6%) are dominant species in this layer. Estimated species similarity of groups found in the litter and humus layers it was found that it is very high and amounted to over 77%. On the basis of the investigations conducted so far it may be stated that the fauna of mites found in the Bucegi Massif is very diverse and distinctly diverges from that shown e.g. in the Polish part of the Carpathian Mountains [Skorupski and Gwiazdowicz 1996, Gwiazdowicz and Sznajdrowski 2000, Gwiazdowicz and Cichocki 2002]. Some species, such as e.g. Pergamasus athiasae, Pergamasus laetus and Leptogamasus doinae have so far been found only in Romania. ACKNOWLEDGEMENT The authors would like to thank Dr. Honciuc Viorica and Dr. Ilinca Juvara-Balş for their help during collection and identification of mites. REFERENCES Fenda P., Mašan P., 2003. Roztoče Acari (Parasitiformes, ex. Uropodina). In: Acarchnids of the Poloniny National Park. Eds. P. Mašan, J. Svatoň. Štatna ochrana prirody SR Banska Bystrica a Sprava Narodneho parku Poloniny, Snina, 143-205. Gwiazdowicz D.J. Cichocki W., 2002. Wstępne badania nad fauną roztoczy (Acari, Gamasida) okolic Zakopanego. In: Przemiany środowiska przyrodniczego Tatr. Eds W. Borowiec, A. Kotarba, A. Kownacki, Z. Krzan, Z. Mirek. Tat. Park Nar., Pol. Tow. Przyj. Nauk Ziem, Kraków, 265-269. Gwiazdowicz D.J., Sznajdrowski R., 2000. Mites (Acari, Gamasida) from selected microhabitats of Bieszczady National Park. Mater. 26 Symp. Akarologicznego Acarologia Polska u progu nowego tysiąclecia, Kazimierz Dolny, 98-109. Honciuc V., Stanescu M., 2003. Acari Fauna (Mesostigmata; Prostigmata; Oribatida) from Piatra Craiului National Park. Researches in Piatra Craiului National Park, 1, 159-170. Juvara-Bals I., 1974. Sur le Leptogamasus de Roumanie. Sous- genre Ernogamasus Athias (Acarina, Anactinotrichida, Parasitina). Trav. Inst. Speol. Emile Racovitza, 13, 23-35. Juvara-Bals I., 1976. Contribution a la connaissance des genres Pergamasus Berlese et Amblygamasus Berlese de Roumanie. Trav. Inst. Speol. Emile Racovitza, 15, 17-52. Mašan P., Fenda P., 2003. Roztoče Acari (Parasitiformes, Uropodina). In: Acarchnids of the Poloniny National Park. Eds. P. Mašan, J. Svatoň. Štatna ochrana prirody SR Banska Bystrica a Sprava Narodneho parku Poloniny, Snina, 207-231. Skorupski M., Gwiazdowicz D.J., 1996. Roztocze (Acari, Mesostigmata) Pienin. Fragm. Faun. 39 (16), 223-243. Silvarum Colendarum Ratio et Industria Lignaria 3(2) 2004

84 M. Stănescu, D.J. Gwiazdowicz WSTĘPNE BADANIA NAD ROZTOCZAMI Z RZĘDU MESOSTIGMATA (ACARI) Z DRZEWOSTANÓW ŚWIERKOWYCH W BUCEGI MASSIF W RUMUNII Streszczenie. Przedstawiono wstępne wyniki badań nad fauną roztoczy wykazanych w drzewostanach świerkowych Bucegi Massif w środkowej Rumunii. W zebranym materiale wyodrębniono 6436 osobników, które zaklasyfikowano do 62 gatunków z 15 rodzin. Najliczniej występowały takie gatunki, jak Veigaia nemorensis (1660 osobników), Leptogamasus parvulus (804) i Leptogamasus tectegynellus (694), z kolei w największej liczbie prób odnotowano: Veigaia nemorensis (224 prób), Neopodocinum mrciaki (150) i Pachyseius humeralis (150). W warstwie ściółki wykazano 4842 osobniki roztoczy należących do 54 gatunków, a w warstwie humusu 1594 osobniki reprezentujące 47 gatunków. Stwierdzono bardzo wysokie podobieństwo gatunkowe zgrupowań występujących w warstwie ściółki i warstwie humusu jego wskaźnik wynosi ponad 77%. Słowa kluczowe: roztocze, Acari, Mesostigmata, ściółka, Bucegi Massif, Rumunia Accepted for print Zaakceptowano do druku: 27.10.2004