Corynocera ambigua (Insecta, Diptera) subfossils occurrence in recent sediments of four shallow Polesie lakes

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.2478/v67-8-16-z annales universitatis mariae curie-skłodowska lublin polonia VOL. LXIII, 2 SECTIO C 8 ANNA HALKIEWICZ Department of Hydrobiology, University of Life Sciences in Lublin, Dobrzańskiego 37, -262 Lublin, e-mail: anna.halkiewicz@up.lublin.pl Corynocera ambigua (Insecta, Diptera) subfossils occurrence in recent sediments of four shallow Polesie lakes Historia występowania subfosylnych szczątków Corynocera ambigua (Insecta, Diptera) we współczesnych osadach płytkich jezior Polesia SUMMARY Sediment cores were analysed from five shallow Polesie lakes (Eastern Poland) representing trophic status from mezo to hypertrophy. The subfossil remains of Corynocera ambigua ZETTERSTEDT 1837 were found in all the lakes, except for hypertrophic Lake Syczyńskie. The age of the cores was between about years. The most abundant development of Corynocera in the lakes took place around years ago. After a few decades, a sudden drop in the numbers was marked in all the lakes. It was a sign of the further, steady diminishing of the populations, until possible total extinction in the lakes Kleszczów, Głębokie Uścimowskie and Sumin. Only in the mesotrophic Lake Rotcze the remains were present up to the top layers of the sediments. It may suggest that the species is still present in the lake. STRESZCZENIE Analizowano rdzenie osadów z pięciu płytkich jezior poleskich zróżnicowanych pod względem statusu troficznego od mezo- po hypertrofię. Sybfosylne szczątki C. ambigua stwierdzono we wszystkich jeziorach z wyjątkiem hypertroficznego jeziora Syczyńskiego. Wiek analizowanych osadów sięgał od ok. 2 do ponad lat. Najsilniejszy rozwój Corynocera w jeziorach miał miejsce ok. lat temu. Następnie we wszystkich jeziorach doszło do dość gwałtownego spadku liczebności, co było zapowiedzią dalszego stopniowego spadku populacji aż do jej prawdopodobnego całkowitego wyginięcia w jeziorach Kleszczów, Głębokie Uścimowskie i Sumin. Tylko w mezotroficznym jeziorze Rotcze szczątki były obecne aż do stropowej części rdzenia, co może sugerować obecność tego gatunku we współczesnej żyjącej faunie. K e y w o r d s: subfossil chironomids, Corynocera ambigua, shallow lakes

32 ANNA HALKIEWICZ INTRODUCTION C. ambigua has attracted attention of paleolimnologists for a long time (e.g. 3, 2). In older literature it was believed to be a cold-stenothermal species living in the sediments of shallow, oligotrophic lakes of arctic and subarctic regions (6, 14, ). In areas located more to the south, it was considered a postglacial relict (16). It was also found in late-glacial sediments of temperate lakes, often in very high numbers (3). However, in recent years, information about the occurrence of living larvae in temperate lakes has been reported (3). This questions its bioindicative value as a cold-stenotherm. In Poland, living larvae of the species were also encountered in several shallow, macrophytedominated lakes located in the northern part of the country (23). Subfossil remalns of C. ambigua were found first time in Poland in Lublin Polesie lakes. This paper presents the occurrence and dynamics in C. ambiqua numbers in the period of the recent centuries. STUDY AREA The research was carried out in five lakes: Kleszczów, Rotcze, Sumin, Głębokie Uścimowskie and Syczyńskie), located in the Łęczna-Włodawa Lakeland (51 o 3 N, 23 o E); (Fig. 1). They are relatively shallow (2.9 6.4 m), but differ in respect of area (6-5 ha) and trophic status (from meso to hypertrophy) (Tab. 1). Their emergent vegetation is similar, forming a belt of various widths, composed mainly of reed Phragmites australis. In contrast, the submerged macrophytes differ between the lakes with regard to their qualitative and quantitative composition (PVI = % 34.5%). In mesotrophic lakes Kleszczów and Rotcze, they form extensive underwater meadows; in eutrophic Głębokie and Sumin, they occur in scattered clumps; and in hypertrophic Lake Syczyńskie, they are almost deprived (Tab.1). According to the theory of alternative stable states of lakes (17), the mesotrophic lakes Kleszczów and Rotcze are classified as macrophyte-dominated, hypertrophic Lake Syczyńskie as phytoplankton-dominated, and the remaining lakes Sumin and Głębokie Uścimowskie represent intermediate status (). For years, these lakes have been an object of limnological research taking into consideration physico-chemical properties of water and bottom sediments (15, 18, 19) and dwelling biocoenosis (e.g. 5, 13,, 12, 9). Tab. 1. Morphometric characteristics of the lakes, Secchi Disc visibility (SD), submerged macrophytes development, expressed as PVI coefficient (percent of lake water volume infested), and trophic status (according to Kornijów et al. 2). Lake Surface area [ha] Max. depth [m] SD [m] PVI [%] Trophic status Kleszczów 5 3 2.4 29.3 mesotrophy Rotcze 46 4.3 2.5 34.5 mesotrophy Sumin 86 6.5 1.6 3.3 eutrophy Głębokie U. 6.4.9.75 eutrophy Syczyńskie 6 2.9.3 hypertrophy

Corynocera ambigua (Insecta, Diptera) subfossils occurrence in recent sediments 33 Fig. 1. Location of the lakes studied in the Łęczna-Włodawa Lakeland METHODS The research was carried out in 5, right after the disappearance of ice. Sediment cores of.4 m.7 m in length were taken from the deepest part of the lakes using a gravitational Uwiteccorer equipped with a plexiglas tube of 1 cm in length and 6 cm in diameter. The cores were sliced into 1 cm layers. Until the depth of cm, each slice was analysed, below that every fifth. The samples were treated following the procedures by Warwick (21). The subfossil material was sieved through a 18-µm screen. The head capsules were identified according to Brooks et al. (4) and Wiederholm (22). The numbers of head capsules (HC) were converted into g dry weight of the sediments. The age of the sediments was calculated on the basis of a constant rate of supply of unsupported 2 Pb model (1) by Gąsiorowski (7). RESULTS The remains of C. ambigua were found in four out of five examined lakes. Only in the hypertrophic, phytoplankton-dominated Lake Syczyńskie, they were not encountered. The highest numbers of head capsules, although differing in individual lakes (range: 189 771 HC/ g DW), were recorded above years ago (Fig. 2). There was a drop in the numbers of midge towards the sediment top in all the lakes, except Lake Rotcze. In the lakes Kleszczów and Głębokie Uścimowskie, it even became extinct on the level of 8 and 7 cm, respectively (Fig. 2). The de-

34 ANNA HALKIEWICZ 2Pb dates 2 Pbdates 2Pb dates 2 4 L. ROTCZE 2 Pbdates 1991 6 8 1985 12 14 16 15 18 22 24 26 25 28 3 3 32 34 36 35 38 42 44 46 45 48 1835 5 1855 5 1815 52 54 56 55 58 6 62 6 64 66 65 68 7 6 8 HC/ gdw HC/ gdw 5 L. SUMIN L. KLESZCZÓW L. GLĘBOKIE U. Fig. 2. Changes in Corynocera ambigua numbers in four Polesie Lakes sediment profiles. In the hypertrophic Lake Syczyńskie C. ambigua was not found 8 12 14 16 18 22 24 26 28 3 32 34 36 38 42 44 46 48 5 52 54 56 58 6 2 4 6 HC/ gdw 1993 1855 12 14 16 18 22 24 26 28 3 32 34 36 38 2 4 6 8 3 HC/ gdw

Corynocera ambigua (Insecta, Diptera) subfossils occurrence in recent sediments 35 cline was quite sudden in all the lakes and took place in approximately the same period, around 15 19 years ago. The increase in the numbers of head capsules took place again only in Lake Rotcze, in the beginning of the 199s. (Fig. 2). DISCUSSION AND CONCLUSIONS Corynocera was found in meso-, as well as in eutrophic Polesie lakes. It is in agreement with the records of Brodersen and Lindegaard (3), who found the species in a broad spectrum of lake fertility, from oligo to eutrophy. The most abundant development of C. ambigua in the lakes studied took place around years ago. Then, the numbers of the midge rapidly dropped. Taking into consideration that the decline took place in all the lakes in approximately the same period, it can be assumed that it was brought about by the same external reason. In Danish Lake Stigsholm, a decline in Corynocera abundance in the sediments, although not so sudden, took place in approximately the same years (3). In the lakes Głębokie Uścimowskie, Kleszczów and Sumin, the species most probably became extinct. It is interesting that in the two very similar lakes (Kleszczów and Rotcze), the fate of Corynocera was different. It vanished around 1985 in the former lake, while in the latter one the remains are present until the top sediment layers. This raises the question whether the larvae of Corynocera still live in Lake Rotcze. In order to answer this question it is hecessary to carry out limnological analysis of currently living Chironomid larvae in the lake. REFERENCES 1. Appleby P. G. 1. Chronostratigraphic techniques in recent sediments. [In:] Tracking Environmental Changes Using Lake Sediments. Last W. M., Smol J. P. (eds), Vol. 1: Kluwer Academic Publishers, Dordrecht, 171 3. 2. Ashe P., McCarthy T. K., Fay Sharon.. The supposed relict Corynocera ambigua Zetterstedt in Ireland (Diptera: Chironomidae), with a review of its ecology and distribution. Verh. Internat. Verein. Limnol. 27: 267 272. 3. Brodersen K. P., Lindegaard C. 1999. Mass occurrence and sporadic distribution of Corynocera ambigua Zettersted (Diptera, Chironomidae) in Danish lakes. Neo- and paleolimnological records. J. Paleolimnology. 22: 41 52. 4. Brooks S. J., Langdon P. G., Heiri O. 7. The Identification and Use of Palearctic Chironomidae Larvae in Paleoecology. Quaternary Research Association, London. 5. Fijałkowski D. 196. Szata roślinna jezior łęczyńsko-włodawskich i otaczających je torfowisk. Vegetation of Łęczna-Włodawa lakes and surrounding them pit-bogs. Ann. UMCS, sectio B 14: 131 6. 6. Fjellberg A. 1972. Present and Late Weichselian occurrence of Corynocera ambigua Zett. (Dipt., Chironomidae) in Norway. Norsk Ent. Tidsskr. 19: 59 61.

36 ANNA HALKIEWICZ 7. Gąsiorowski M. Deposition rate of sediments under different alternative stable states. Geochronologia. In press. 8. Kornijów R., Halkiewicz A. 7. Uwarunkowania zaburzeń sekwencji odkładania osadów dennych w płytkich jeziorach poleskich w kontekście ich przydatności do badań paleoekologicznych. Possible disturbance of sedimentation in shallow Polesie lakes considered from the view point of their suitability for paleoecological studies. St. Limnol. Telmat. 1: 83 86. 9. Kornijów R., Pęczuła W. 5. Ecosystem of a small and shallow lake suffering from cyanobacterial blooms hypertrophic, phytoplankton dominated or both? Verh. Internat. Verein. Limnol. 29: 15 19.. Kornijów R., Pęczuła W., Lorens B., Ligęza S., Rechulicz J., Kowalczyk-Pecka D. 2. Shallow Polesie Lakes from the view point of the alternative stable states theory. Acta Agrophysica. 68: 61 72. 11. Kornijów R., Radwan S., Kowalik W., Kowalczyk C., Wojciechowska W. 1995. Zmiany jakościowe i ilościowe w biocenozach jezior Poleskiego Parku Narodowego i jego otuliny w latach 1955 1994. [W:] Radwan S. (red.) Ochrona ekosystemów wodnych w Poleskim Parku Narodowym i jego otulinie. Qualitative and quantitative changes in biocenosis of Polesie National Park lakes and its buffer zone in the years 1955 1994. [In:] Radwan S. (ed.) Protection of water ecosystems in the lakes of Polesie National Park and its buffer zone. AR Lublin, TWWP Lublin, 63 68. 12. Kornijów R., Rechulicz J., Halkiewicz A. 3. Brown bullhead (Ictalurus nebulosus Le Sueur) in ichthyofauna of several Polesie lakes differing in trophic status. Acta Scientiarum Polonorum, Piscaria 2: 131 139. 13. Kowalczyk C., 1977. Fauna skorupiaków jezior Pojezierza Łęczyńsko-Włodawskiego na tle warunków limnologicznych. Część I: Jeziora o I i II stopniu degradacji. The microcrustacean fauna of Łęczna-Włodawa Lakeland on the background of limnological conditions. Part I: The lakes of I and II degree of degradation. Ann. UMCS, sec. C, 32: 293 322. 14. Pinder L. C. V., Reiss F. 1983. The larvae of Chironominae (Diptera: Chironomidae) of Holarctic region keys and diagnoses. In: Chironomidae of the Holarctic region. Keys and diagnoses. Part 1 Larvae. Wiederholm T. (ed.), Ent. Scand., Suppl. 19: 293 435. 15. Radwan S., Kowalczyk C., Podgórski W., Fall J. 1973. Materiały do hydrochemii Pojezierza Łęczyńsko-Włodawskiego. Część III: Właściwości fizyczne i chemiczne. Materials to hydrochemistry of Łęczna-Włodawa Lakeland. Part III: Physical and chemical properties. Ann. UMCS, sectio C 28: 97 116. 16. Reiss F., Gerstmeier R. 1984. Corynocera ambigua Zetterstedt als Glazialrelikt im Starnberger See, Oberbayern. Nachrichtenblatt der Bayersichen Entomol. 33: 58 61. 17. Scheffer M., Hosper S.,H., Meijer M.L., Moss B., Jeppesen E. 1993. Alternative equilibria in shallow lakes. TREE. 8: 275 279. 18. Smal H., Kornijów R., Ligęza S. 5. The effect of catchment on water quality and eutrophication risk of five shallow lakes (Polesie Region, Eastern Poland). Pol. J. Ecol. 53: 313 327. 19. Szafran K., Misztal M. 6. The chemical composition of bottom sediments of two shallow eastern Polish lakes with different submerged macrophytes biomass. Verh. Internat. Verein. Limnol. 29: 35 38.. Walker I. R., Mathewes R. W. 1988. Late Quaternary fossil Chironomidae (Diptera) from Hippa Lake, Queen Charlotte Islands, British Columbia, with special reference to Corynocera Zett. Can. Ent. 1: 739 751. 21. Warwick W. F. 198. Paleolimnology of the Bay of Quinte, Lake Ontario: 28 years of cultural influence. Can. Bull. Fish. Aq. Sci. 6: 1 117. 22. Wiederholm T. 1983. Chironomidae of the Holarctic region: keys and diagnoses. Part 1. Larvae. Entomol. Scand. Suppl. 19: 1 457. 23. Żbikowski J., Kobak J. 7. Factors influencing taxonomic composition and abundance of macrozoobenthos in extralittoral zone of shallow eutrophic lakes. Hydrobiologia. 584: 145 155.